Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles
Copyright © 2018 Elsevier B.V. All rights reserved..
Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0-2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:251 |
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| Enthalten in: |
Virus research - 251(2018) vom: 02. Juni, Seite 1-5 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Melegari, Irene [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 21.09.2018 Date Revised 21.09.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virusres.2018.04.014 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM283463007 |
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| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0-2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age | ||
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| 650 | 4 | |a Canine sapoviruses | |
| 650 | 4 | |a Dogs | |
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| 650 | 4 | |a Virus like-particles | |
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| 700 | 1 | |a Marsilio, Fulvio |e verfasserin |4 aut | |
| 700 | 1 | |a Di Profio, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Sarchese, Vittorio |e verfasserin |4 aut | |
| 700 | 1 | |a Massirio, Ivano |e verfasserin |4 aut | |
| 700 | 1 | |a Palombieri, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a D'Angelo, Anna Rita |e verfasserin |4 aut | |
| 700 | 1 | |a Lanave, Gianvito |e verfasserin |4 aut | |
| 700 | 1 | |a Diakoudi, Georgia |e verfasserin |4 aut | |
| 700 | 1 | |a Cavalli, Alessandra |e verfasserin |4 aut | |
| 700 | 1 | |a Martella, Vito |e verfasserin |4 aut | |
| 700 | 1 | |a Di Martino, Barbara |e verfasserin |4 aut | |
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