Structure and gene cluster of the O-antigen of Escherichia coli O54
Copyright © 2018 Elsevier Ltd. All rights reserved..
Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O54 afforded an O-polysaccharide, which was studied by sugar analysis, solvolysis with anhydrous trifluoroacetic acid, and 1H and 13C NMR spectroscopy. Solvolysis cleaved predominantly the linkage of β-d-Ribf and, to a lesser extent, that of β-d-GlcpNAc, whereas the other linkages, including the linkage of α-l-Rhap, were stable under selected conditions (40 °C, 5 h). The following structure of the O-polysaccharide was established: →4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1 → 2)-β-d-Ribf-(1 → 4)-β-d-Galp-(1 → 3)-β-d-GlcpNAc-(1→ The O-antigen gene cluster of E. coli O54 was analyzed and found to be consistent in general with the O-polysaccharide structure established but there were two exceptions: i) in the cluster, there were genes for phosphoserine phosphatase and serine transferase, which have no apparent role in the O-polysaccharide synthesis, and ii) no ribofuranosyltransferase gene was present in the cluster. Both uncommon features are shared by some other enteric bacteria.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:462 |
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| Enthalten in: |
Carbohydrate research - 462(2018) vom: 15. Juni, Seite 34-38 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Naumenko, Olesya I [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial polysaccharide structure |
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| Anmerkungen: |
Date Completed 24.09.2018 Date Revised 24.09.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carres.2018.04.001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM283089172 |
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| 500 | |a Date Revised 24.09.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O54 afforded an O-polysaccharide, which was studied by sugar analysis, solvolysis with anhydrous trifluoroacetic acid, and 1H and 13C NMR spectroscopy. Solvolysis cleaved predominantly the linkage of β-d-Ribf and, to a lesser extent, that of β-d-GlcpNAc, whereas the other linkages, including the linkage of α-l-Rhap, were stable under selected conditions (40 °C, 5 h). The following structure of the O-polysaccharide was established: →4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1 → 2)-β-d-Ribf-(1 → 4)-β-d-Galp-(1 → 3)-β-d-GlcpNAc-(1→ The O-antigen gene cluster of E. coli O54 was analyzed and found to be consistent in general with the O-polysaccharide structure established but there were two exceptions: i) in the cluster, there were genes for phosphoserine phosphatase and serine transferase, which have no apparent role in the O-polysaccharide synthesis, and ii) no ribofuranosyltransferase gene was present in the cluster. Both uncommon features are shared by some other enteric bacteria | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bacterial polysaccharide structure | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a O-antigen | |
| 650 | 4 | |a O-antigen gene cluster | |
| 650 | 4 | |a O-polysaccharide | |
| 650 | 7 | |a Escherichia coli Proteins |2 NLM | |
| 650 | 7 | |a O Antigens |2 NLM | |
| 650 | 7 | |a Polysaccharides, Bacterial |2 NLM | |
| 700 | 1 | |a Guo, Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Senchenkova, Sof'ya N |e verfasserin |4 aut | |
| 700 | 1 | |a Geng, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Perepelov, Andrei V |e verfasserin |4 aut | |
| 700 | 1 | |a Shashkov, Alexander S |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Knirel, Yuriy A |e verfasserin |4 aut | |
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