Coordination of Rad1-Rad10 interactions with Msh2-Msh3, Saw1 and RPA is essential for functional 3' non-homologous tail removal
Double strand DNA break repair (DSBR) comprises multiple pathways. A subset of DSBR pathways, including single strand annealing, involve intermediates with 3' non-homologous tails that must be removed to complete repair. In Saccharomyces cerevisiae, Rad1-Rad10 is the structure-specific endonuclease that cleaves the tails in 3' non-homologous tail removal (3' NHTR). Rad1-Rad10 is also an essential component of the nucleotide excision repair (NER) pathway. In both cases, Rad1-Rad10 requires protein partners for recruitment to the relevant DNA intermediate. Msh2-Msh3 and Saw1 recruit Rad1-Rad10 in 3' NHTR; Rad14 recruits Rad1-Rad10 in NER. We created two rad1 separation-of-function alleles, rad1R203A,K205A and rad1R218A; both are defective in 3' NHTR but functional in NER. In vitro, rad1R203A,K205A was impaired at multiple steps in 3' NHTR. The rad1R218A in vivo phenotype resembles that of msh2- or msh3-deleted cells; recruitment of rad1R218A-Rad10 to recombination intermediates is defective. Interactions among rad1R218A-Rad10 and Msh2-Msh3 and Saw1 are altered and rad1R218A-Rad10 interactions with RPA are compromised. We propose a model in which Rad1-Rad10 is recruited and positioned at the recombination intermediate through interactions, between Saw1 and DNA, Rad1-Rad10 and Msh2-Msh3, Saw1 and Msh2-Msh3 and Rad1-Rad10 and RPA. When any of these interactions is altered, 3' NHTR is impaired.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:46 |
|---|---|
| Enthalten in: |
Nucleic acids research - 46(2018), 10 vom: 01. Juni, Seite 5075-5096 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Eichmiller, Robin [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 22.08.2019 Date Revised 02.11.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1093/nar/gky254 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM283083921 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM283083921 | ||
| 003 | DE-627 | ||
| 005 | 20231225035600.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nar/gky254 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0943.xml |
| 035 | |a (DE-627)NLM283083921 | ||
| 035 | |a (NLM)29660012 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Eichmiller, Robin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Coordination of Rad1-Rad10 interactions with Msh2-Msh3, Saw1 and RPA is essential for functional 3' non-homologous tail removal |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.08.2019 | ||
| 500 | |a Date Revised 02.11.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Double strand DNA break repair (DSBR) comprises multiple pathways. A subset of DSBR pathways, including single strand annealing, involve intermediates with 3' non-homologous tails that must be removed to complete repair. In Saccharomyces cerevisiae, Rad1-Rad10 is the structure-specific endonuclease that cleaves the tails in 3' non-homologous tail removal (3' NHTR). Rad1-Rad10 is also an essential component of the nucleotide excision repair (NER) pathway. In both cases, Rad1-Rad10 requires protein partners for recruitment to the relevant DNA intermediate. Msh2-Msh3 and Saw1 recruit Rad1-Rad10 in 3' NHTR; Rad14 recruits Rad1-Rad10 in NER. We created two rad1 separation-of-function alleles, rad1R203A,K205A and rad1R218A; both are defective in 3' NHTR but functional in NER. In vitro, rad1R203A,K205A was impaired at multiple steps in 3' NHTR. The rad1R218A in vivo phenotype resembles that of msh2- or msh3-deleted cells; recruitment of rad1R218A-Rad10 to recombination intermediates is defective. Interactions among rad1R218A-Rad10 and Msh2-Msh3 and Saw1 are altered and rad1R218A-Rad10 interactions with RPA are compromised. We propose a model in which Rad1-Rad10 is recruited and positioned at the recombination intermediate through interactions, between Saw1 and DNA, Rad1-Rad10 and Msh2-Msh3, Saw1 and Msh2-Msh3 and Rad1-Rad10 and RPA. When any of these interactions is altered, 3' NHTR is impaired | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a MSH3 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a MutS Homolog 3 Protein |2 NLM | |
| 650 | 7 | |a RFA1 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a Replication Protein A |2 NLM | |
| 650 | 7 | |a Saccharomyces cerevisiae Proteins |2 NLM | |
| 650 | 7 | |a Saw1 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a Endonucleases |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 650 | 7 | |a RAD1 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 650 | 7 | |a RAD10 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a EC 3.1.30.1 |2 NLM | |
| 650 | 7 | |a Single-Strand Specific DNA and RNA Endonucleases |2 NLM | |
| 650 | 7 | |a EC 3.1.30.1 |2 NLM | |
| 650 | 7 | |a MSH2 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a EC 3.6.1.3 |2 NLM | |
| 650 | 7 | |a MutS Homolog 2 Protein |2 NLM | |
| 650 | 7 | |a EC 3.6.1.3 |2 NLM | |
| 650 | 7 | |a DNA Repair Enzymes |2 NLM | |
| 650 | 7 | |a EC 6.5.1.- |2 NLM | |
| 700 | 1 | |a Medina-Rivera, Melisa |e verfasserin |4 aut | |
| 700 | 1 | |a DeSanto, Rachel |e verfasserin |4 aut | |
| 700 | 1 | |a Minca, Eugen |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Christopher |e verfasserin |4 aut | |
| 700 | 1 | |a Holland, Cory |e verfasserin |4 aut | |
| 700 | 1 | |a Seol, Ja-Hwan |e verfasserin |4 aut | |
| 700 | 1 | |a Schmit, Megan |e verfasserin |4 aut | |
| 700 | 1 | |a Oramus, Diane |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Jessica |e verfasserin |4 aut | |
| 700 | 1 | |a Gallardo, Ignacio F |e verfasserin |4 aut | |
| 700 | 1 | |a Finkelstein, Ilya J |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sang Eun |e verfasserin |4 aut | |
| 700 | 1 | |a Surtees, Jennifer A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nucleic acids research |d 1974 |g 46(2018), 10 vom: 01. Juni, Seite 5075-5096 |w (DE-627)NLM000063398 |x 1362-4962 |7 nnns |
| 773 | 1 | 8 | |g volume:46 |g year:2018 |g number:10 |g day:01 |g month:06 |g pages:5075-5096 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/nar/gky254 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 46 |j 2018 |e 10 |b 01 |c 06 |h 5075-5096 | ||