Modulating the protein content of complex proteomes using acetonitrile
Copyright © 2018 Elsevier B.V. All rights reserved..
In this work we present acetonitrile as a tool to modulate the dynamic range of the proteome of complex samples. Different concentrations of acetonitrile ranging from 15% v/v to 65% v/v were used to modulate the protein content of serum samples from healthy people and patients with lymphoma and myeloma. We show that the proteome above 70 kDa is pelleted as a function of the concentration of acetonitrile and that profiling with PCA or Clustering is only possible using the supernatants obtained for concentrations of acetonitrile higher than 45% v/v or the pellets for concentrations of acetonitrile of 35% and 45%. The differentiation and classification of the three groups of sera samples (healthy, lymphoma and myeloma) were possible using acetonitrile at 55% v/v concentration. This work opens new avenues for the application of acetonitrile as a cost-effective tool in proteomics applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:182 |
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| Enthalten in: |
Talanta - 182(2018) vom: 15. Mai, Seite 333-339 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prates, João [VerfasserIn] |
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| Links: |
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| Themen: |
Acetonitrile |
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| Anmerkungen: |
Date Completed 07.09.2018 Date Revised 07.09.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2018.01.057 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM28158303X |
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| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a In this work we present acetonitrile as a tool to modulate the dynamic range of the proteome of complex samples. Different concentrations of acetonitrile ranging from 15% v/v to 65% v/v were used to modulate the protein content of serum samples from healthy people and patients with lymphoma and myeloma. We show that the proteome above 70 kDa is pelleted as a function of the concentration of acetonitrile and that profiling with PCA or Clustering is only possible using the supernatants obtained for concentrations of acetonitrile higher than 45% v/v or the pellets for concentrations of acetonitrile of 35% and 45%. The differentiation and classification of the three groups of sera samples (healthy, lymphoma and myeloma) were possible using acetonitrile at 55% v/v concentration. This work opens new avenues for the application of acetonitrile as a cost-effective tool in proteomics applications | ||
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| 650 | 4 | |a Disease | |
| 650 | 4 | |a Method | |
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| 700 | 1 | |a López-Fernández, Hugo |e verfasserin |4 aut | |
| 700 | 1 | |a Lodeiro, Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Capelo, J L |e verfasserin |4 aut | |
| 700 | 1 | |a Santos, Hugo M |e verfasserin |4 aut | |
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