Blood-brain barrier disruption induced by diagnostic ultrasound combined with microbubbles in mice
OBJECTIVE: To investigate the effects of the microbubble (MB) dose, mechanism index (MI) and sonication duration on blood-brain barrier (BBB) disruption induced by diagnostic ultrasound combined with MBs as well as to investigate the potential molecular mechanism.
RESULTS: The extent of BBB disruption increased with MB dose, MI and sonication duration. A relatively larger extent of BBB disruption associated with minimal tissue damage was achieved by an appropriate MB dose and ultrasound exposure parameters with diagnostic ultrasound. Decreased expression of ZO-1, occludin and claudin-5 were correlated with disruption of the BBB, as confirmed by paracellular passage of the tracer lanthanum nitrate into the brain parenchyma after BBB disruption.
CONCLUSIONS: These findings indicated that this technique is a promising tool for promoting brain delivery of diagnostic and therapeutic agents in the diagnosis and treatment of brain diseases.
METHODS: The extent of BBB disruption was qualitatively assessed by Evans blue (EB) staining and quantitatively analyzed by an EB extravasation measurement. A histological examination was performed to evaluate tissue damage. Expression of tight junction (TJ) related proteins ZO-1, occludin and claudin-5 was determined by western blotting analysis and immunohistofluorescence. Transmission electron microscopy was performed to observe ultrastructure changes of TJs after BBB disruption.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Oncotarget - 9(2018), 4 vom: 12. Jan., Seite 4897-4914 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Bingxia [VerfasserIn] |
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| Links: |
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| Themen: |
Blood-brain barrier |
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| Anmerkungen: |
Date Revised 14.03.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.18632/oncotarget.23527 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM280941587 |
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| 100 | 1 | |a Zhao, Bingxia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Blood-brain barrier disruption induced by diagnostic ultrasound combined with microbubbles in mice |
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| 500 | |a Date Revised 14.03.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a OBJECTIVE: To investigate the effects of the microbubble (MB) dose, mechanism index (MI) and sonication duration on blood-brain barrier (BBB) disruption induced by diagnostic ultrasound combined with MBs as well as to investigate the potential molecular mechanism | ||
| 520 | |a RESULTS: The extent of BBB disruption increased with MB dose, MI and sonication duration. A relatively larger extent of BBB disruption associated with minimal tissue damage was achieved by an appropriate MB dose and ultrasound exposure parameters with diagnostic ultrasound. Decreased expression of ZO-1, occludin and claudin-5 were correlated with disruption of the BBB, as confirmed by paracellular passage of the tracer lanthanum nitrate into the brain parenchyma after BBB disruption | ||
| 520 | |a CONCLUSIONS: These findings indicated that this technique is a promising tool for promoting brain delivery of diagnostic and therapeutic agents in the diagnosis and treatment of brain diseases | ||
| 520 | |a METHODS: The extent of BBB disruption was qualitatively assessed by Evans blue (EB) staining and quantitatively analyzed by an EB extravasation measurement. A histological examination was performed to evaluate tissue damage. Expression of tight junction (TJ) related proteins ZO-1, occludin and claudin-5 was determined by western blotting analysis and immunohistofluorescence. Transmission electron microscopy was performed to observe ultrastructure changes of TJs after BBB disruption | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a diagnostic ultrasound | |
| 650 | 4 | |a drug delivery | |
| 650 | 4 | |a microbubble | |
| 700 | 1 | |a Chen, Yihan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jinfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yali |e verfasserin |4 aut | |
| 700 | 1 | |a Lv, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Mingxing |e verfasserin |4 aut | |
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