Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of soluble and membranous proteins at various buffer conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Scientific reports - 8(2018), 1 vom: 23. Jan., Seite 1457 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hoeser, Jo [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 22.10.2018 Date Revised 23.01.2019 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1038/s41598-018-19702-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM280239602 |
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| 520 | |a Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of soluble and membranous proteins at various buffer conditions | ||
| 650 | 4 | |a Journal Article | |
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