Enhanced gene delivery by polyethyleneimine coated mesoporous silica nanoparticles
Due to large surface area, tunable pore size, easy surface manipulation, and low-toxicity mesoporous silica nanoparticles (MSNs) may act as a suitable vector for gene delivery. In order to make MSNs as a suitable gene delivery system, we modified the surface of phosphonated MSNs (PMSN) with polyethyleneimine (PEI) 10 and 25 KDa. Then nanoparticles were loaded with chloroquine (CQ) (a lysosomotropic agent) and complexed with plasmid DNA. The transfection efficiency and cytotoxicity of these nanoparticles was examined using green fluorescent protein plasmid (pGFP) and cytotoxicity assay. All PEI coated nanoparticles showed positive zeta potential and mean size was ranged between 170 and 215 nm with polydispersity index bellow 0.35. PEI-coated MSNs significiantly enhanced GFP gene expression in Neuro-2 A cells compared to PEI 10 and 25 KDa. The results of the cytotoxicity assays showed that these nanoparticles have an acceptable level of viability but CQ loaded nanoparticles showed higher cytotoxicity and lower transfection activity than CQ free nanoparticles.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
Pharmaceutical development and technology - 24(2019), 1 vom: 20. Jan., Seite 127-132 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zarei, Hassan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 08.02.2019 Date Revised 15.02.2019 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1080/10837450.2018.1431930 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM280193726 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM280193726 | ||
| 003 | DE-627 | ||
| 005 | 20231225024753.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/10837450.2018.1431930 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0933.xml |
| 035 | |a (DE-627)NLM280193726 | ||
| 035 | |a (NLM)29357725 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zarei, Hassan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Enhanced gene delivery by polyethyleneimine coated mesoporous silica nanoparticles |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.02.2019 | ||
| 500 | |a Date Revised 15.02.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Due to large surface area, tunable pore size, easy surface manipulation, and low-toxicity mesoporous silica nanoparticles (MSNs) may act as a suitable vector for gene delivery. In order to make MSNs as a suitable gene delivery system, we modified the surface of phosphonated MSNs (PMSN) with polyethyleneimine (PEI) 10 and 25 KDa. Then nanoparticles were loaded with chloroquine (CQ) (a lysosomotropic agent) and complexed with plasmid DNA. The transfection efficiency and cytotoxicity of these nanoparticles was examined using green fluorescent protein plasmid (pGFP) and cytotoxicity assay. All PEI coated nanoparticles showed positive zeta potential and mean size was ranged between 170 and 215 nm with polydispersity index bellow 0.35. PEI-coated MSNs significiantly enhanced GFP gene expression in Neuro-2 A cells compared to PEI 10 and 25 KDa. The results of the cytotoxicity assays showed that these nanoparticles have an acceptable level of viability but CQ loaded nanoparticles showed higher cytotoxicity and lower transfection activity than CQ free nanoparticles | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chloroquine | |
| 650 | 4 | |a cytotoxicity | |
| 650 | 4 | |a mesoporous silica nanoparticle | |
| 650 | 4 | |a polyethyleneimine | |
| 650 | 4 | |a transfection | |
| 650 | 7 | |a Green Fluorescent Proteins |2 NLM | |
| 650 | 7 | |a 147336-22-9 |2 NLM | |
| 650 | 7 | |a Silicon Dioxide |2 NLM | |
| 650 | 7 | |a 7631-86-9 |2 NLM | |
| 650 | 7 | |a Chloroquine |2 NLM | |
| 650 | 7 | |a 886U3H6UFF |2 NLM | |
| 650 | 7 | |a Polyethyleneimine |2 NLM | |
| 650 | 7 | |a 9002-98-6 |2 NLM | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 700 | 1 | |a Kazemi Oskuee, Reza |e verfasserin |4 aut | |
| 700 | 1 | |a Hanafi-Bojd, Mohammad Yahya |e verfasserin |4 aut | |
| 700 | 1 | |a Gholami, Leila |e verfasserin |4 aut | |
| 700 | 1 | |a Ansari, Legha |e verfasserin |4 aut | |
| 700 | 1 | |a Malaekeh-Nikouei, Bizhan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pharmaceutical development and technology |d 1998 |g 24(2019), 1 vom: 20. Jan., Seite 127-132 |w (DE-627)NLM094740194 |x 1097-9867 |7 nnns |
| 773 | 1 | 8 | |g volume:24 |g year:2019 |g number:1 |g day:20 |g month:01 |g pages:127-132 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/10837450.2018.1431930 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 24 |j 2019 |e 1 |b 20 |c 01 |h 127-132 | ||