Harpagoside-induced anaphylactic reaction in an IgE-independent manner both in vitro and in vivo
BACKGROUND: Harpagoside (HAR) is an active component of Scrophularia ningpoensis (SN), which has anti-inflammatory and anti-immune effects. SN is used widely in China to treat various diseases. Recently, SN has been used as a traditional Chinese medicine injection and used clinically. However, allergic responses to these injections are frequently reported.
AIM: We examined whether the main component of SN, HAR, is associated with the allergic reaction to SN.
METHODS: This study assessed the effects of HAR in mice and mast cell activation to characterize its anaphylactic effects and underlying mechanisms. Mice hindpaw swelling, serum allergy factor detection, enzyme-linked immunosorbent assays, and degranulation assays were performed to measure allergic mediators both in vivo and in vitro.
RESULTS: The present study indicated that HAR induced paw swelling, interleukin-6, inositol triphosphate, tumor necrosis factor-α, and histamine increases in mice. Our in vitro data also showed that HAR induced β-hexosaminidase, inositol triphosphate, and interleukin-6 release, leading to mast cell degranulation. In contrast, neither C48/80 nor HAR induced local anaphylaxis in STOCK KitW-sh/HNihrJaeBsmJNju mice.
CONCLUSIONS: HAR is a potential sensitization compound in SN, and these results provide information for the safe clinical use of SN.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
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| Enthalten in: |
Immunopharmacology and immunotoxicology - 40(2018), 2 vom: 15. Apr., Seite 173-178 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Che, Delu [VerfasserIn] |
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| Links: |
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| Themen: |
37341-29-0 |
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| Anmerkungen: |
Date Completed 29.08.2018 Date Revised 29.08.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/08923973.2018.1424900 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM280167962 |
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| 500 | |a Date Revised 29.08.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Harpagoside (HAR) is an active component of Scrophularia ningpoensis (SN), which has anti-inflammatory and anti-immune effects. SN is used widely in China to treat various diseases. Recently, SN has been used as a traditional Chinese medicine injection and used clinically. However, allergic responses to these injections are frequently reported | ||
| 520 | |a AIM: We examined whether the main component of SN, HAR, is associated with the allergic reaction to SN | ||
| 520 | |a METHODS: This study assessed the effects of HAR in mice and mast cell activation to characterize its anaphylactic effects and underlying mechanisms. Mice hindpaw swelling, serum allergy factor detection, enzyme-linked immunosorbent assays, and degranulation assays were performed to measure allergic mediators both in vivo and in vitro | ||
| 520 | |a RESULTS: The present study indicated that HAR induced paw swelling, interleukin-6, inositol triphosphate, tumor necrosis factor-α, and histamine increases in mice. Our in vitro data also showed that HAR induced β-hexosaminidase, inositol triphosphate, and interleukin-6 release, leading to mast cell degranulation. In contrast, neither C48/80 nor HAR induced local anaphylaxis in STOCK KitW-sh/HNihrJaeBsmJNju mice | ||
| 520 | |a CONCLUSIONS: HAR is a potential sensitization compound in SN, and these results provide information for the safe clinical use of SN | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a IgE-independent | |
| 650 | 4 | |a Scrophularia ningpoensis | |
| 650 | 4 | |a anaphylactoid reaction | |
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| 700 | 1 | |a Hou, Yajing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Nan |e verfasserin |4 aut | |
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