Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma
The role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar intraepithelial neoplasia lesions and vulvar squamous cell carcinoma. The purpose of this study was to select appropriate references for gene expression studies in plasma of patients with these lesions. Expression levels of six microRNAs-hsa-miR-425-5p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-5p, hsa-miR-103a-3p, and hsa-miR-16-5p-were analyzed by quantitative reverse transcription polymerase chain reaction in plasma samples obtained from 17 patients with vulvar intraepithelial neoplasia lesion and 27 patients with vulvar squamous cell carcinoma. The expression stability of these candidate normalizers was assayed using geNorm algorithm. hsa-miR-93-5p was revealed as the most stably expressed reference in plasma samples of both vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients. The results pointed at hsa-miR-93-5p and hsa-miR-425-5p as microRNAs that retained the greatest robustness in plasma of vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients, respectively. Our work is the first report on reference microRNA selection for quantitative real-time polymerase chain reaction applications in vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma. The candidate microRNA stability values for the two types of lesions are provided and might serve for normalization of the future novel microRNA biomarkers in these rare entities.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine - 39(2017), 11 vom: 28. Nov., Seite 1010428317717140 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zalewski, Kamil [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.01.2018 Date Revised 18.01.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1177/1010428317717140 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM279630956 |
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| 100 | 1 | |a Zalewski, Kamil |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar intraepithelial neoplasia lesions and vulvar squamous cell carcinoma. The purpose of this study was to select appropriate references for gene expression studies in plasma of patients with these lesions. Expression levels of six microRNAs-hsa-miR-425-5p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-5p, hsa-miR-103a-3p, and hsa-miR-16-5p-were analyzed by quantitative reverse transcription polymerase chain reaction in plasma samples obtained from 17 patients with vulvar intraepithelial neoplasia lesion and 27 patients with vulvar squamous cell carcinoma. The expression stability of these candidate normalizers was assayed using geNorm algorithm. hsa-miR-93-5p was revealed as the most stably expressed reference in plasma samples of both vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients. The results pointed at hsa-miR-93-5p and hsa-miR-425-5p as microRNAs that retained the greatest robustness in plasma of vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients, respectively. Our work is the first report on reference microRNA selection for quantitative real-time polymerase chain reaction applications in vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma. The candidate microRNA stability values for the two types of lesions are provided and might serve for normalization of the future novel microRNA biomarkers in these rare entities | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Squamous intraepithelial lesions | |
| 650 | 4 | |a microRNA expression | |
| 650 | 4 | |a quantitative real-time polymerase chain reaction | |
| 650 | 4 | |a reference genes | |
| 650 | 4 | |a vulvar squamous cell carcinoma | |
| 650 | 7 | |a Circulating MicroRNA |2 NLM | |
| 650 | 7 | |a MIRN103 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN16 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN191 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN423 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN425 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN93 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a Misiek, Marcin |e verfasserin |4 aut | |
| 700 | 1 | |a Kowalik, Artur |e verfasserin |4 aut | |
| 700 | 1 | |a Bakuła-Zalewska, Elwira |e verfasserin |4 aut | |
| 700 | 1 | |a Kopczyński, Janusz |e verfasserin |4 aut | |
| 700 | 1 | |a Zielińska, Aleksandra |e verfasserin |4 aut | |
| 700 | 1 | |a Bidziński, Mariusz |e verfasserin |4 aut | |
| 700 | 1 | |a Radziszewski, Jakub |e verfasserin |4 aut | |
| 700 | 1 | |a Góźdź, Stanisław |e verfasserin |4 aut | |
| 700 | 1 | |a Kowalewska, Magdalena |e verfasserin |4 aut | |
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