Structural Organization of 6B9 Molecule, a Monoclonal Antibody Against Lycopene
Full cDNA and corresponding amino acid (AA) sequences of 6B9 monoclonal antibody (mAb) against lycopene was obtained using Step-Out RACE technology. Variable (V) and constant (C) regions were identified. The light chain of 6B9 contained 238 AA IgM with the highest level of identity (0.93) to both the anti-VEGF receptor antibody and anti-collagen type II FAb CIIC1. The heavy chain was composed of 634 AA with a high identity (0.9) to the Ig mu chain C region. Potential posttranslational modification regions in both chains were identified alongside with disulfide bond sites. The obtained information can be used for making chimeric constructs containing 6B9 mAb (or its fragments) and lycopene, a powerful carotenoid with antioxidant as well as antiproliferating properties, which can be implemented in the treatment of an aggressive form of prostate cancer and possibly other malignancies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Monoclonal antibodies in immunodiagnosis and immunotherapy - 36(2017), 6 vom: 15. Dez., Seite 259-263 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Petyaev, Ivan M [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.07.2018 Date Revised 02.12.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1089/mab.2017.0041 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM279308795 |
|---|
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| 500 | |a Date Completed 23.07.2018 | ||
| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Full cDNA and corresponding amino acid (AA) sequences of 6B9 monoclonal antibody (mAb) against lycopene was obtained using Step-Out RACE technology. Variable (V) and constant (C) regions were identified. The light chain of 6B9 contained 238 AA IgM with the highest level of identity (0.93) to both the anti-VEGF receptor antibody and anti-collagen type II FAb CIIC1. The heavy chain was composed of 634 AA with a high identity (0.9) to the Ig mu chain C region. Potential posttranslational modification regions in both chains were identified alongside with disulfide bond sites. The obtained information can be used for making chimeric constructs containing 6B9 mAb (or its fragments) and lycopene, a powerful carotenoid with antioxidant as well as antiproliferating properties, which can be implemented in the treatment of an aggressive form of prostate cancer and possibly other malignancies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a immunotherapy | |
| 650 | 4 | |a lycopene | |
| 650 | 4 | |a molecular structure | |
| 650 | 4 | |a monoclonal antibody | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Disulfides |2 NLM | |
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| 650 | 7 | |a Immunoglobulin Light Chains |2 NLM | |
| 650 | 7 | |a Immunoglobulin Variable Region |2 NLM | |
| 650 | 7 | |a Carotenoids |2 NLM | |
| 650 | 7 | |a 36-88-4 |2 NLM | |
| 650 | 7 | |a Lycopene |2 NLM | |
| 650 | 7 | |a SB0N2N0WV6 |2 NLM | |
| 700 | 1 | |a Alekseev, Konstantin P |e verfasserin |4 aut | |
| 700 | 1 | |a Tsibezov, Valeriy V |e verfasserin |4 aut | |
| 700 | 1 | |a Kostina, Ludmila V |e verfasserin |4 aut | |
| 700 | 1 | |a Kozlov, Alexey Y |e verfasserin |4 aut | |
| 700 | 1 | |a Kyle, Nigel H |e verfasserin |4 aut | |
| 700 | 1 | |a Bashmakov, Yuriy K |e verfasserin |4 aut | |
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