Structure and genetics of the O-specific polysaccharide of Escherichia coli O27
Copyright © 2017 Elsevier Ltd. All rights reserved..
The O-specific polysaccharide (O-antigen) is a part of the lipopolysaccharide on the cell surface of Gram-negative bacteria. The O-polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O27 and studied by sugar analysis and Smith degradation along with 1H and 13C NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established, which is unique among known structures of bacterial polysaccharides:where GlcA is non-stoichiometrically O-acetylated at position 3 (∼22%) or 4 (∼37%). Functions of genes in the O-antigen gene cluster of E. coli O27 were tentatively assigned by comparison with sequences in the available databases and found to be consistent with the O-polysaccharide structure.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:456 |
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| Enthalten in: |
Carbohydrate research - 456(2018) vom: 01. Feb., Seite 1-4 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Perepelov, Andrei V [VerfasserIn] |
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| Links: |
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| Themen: |
Escherichia coli |
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| Anmerkungen: |
Date Completed 24.09.2018 Date Revised 24.09.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carres.2017.11.016 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM278856675 |
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| 500 | |a Date Completed 24.09.2018 | ||
| 500 | |a Date Revised 24.09.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2017 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The O-specific polysaccharide (O-antigen) is a part of the lipopolysaccharide on the cell surface of Gram-negative bacteria. The O-polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O27 and studied by sugar analysis and Smith degradation along with 1H and 13C NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established, which is unique among known structures of bacterial polysaccharides:where GlcA is non-stoichiometrically O-acetylated at position 3 (∼22%) or 4 (∼37%). Functions of genes in the O-antigen gene cluster of E. coli O27 were tentatively assigned by comparison with sequences in the available databases and found to be consistent with the O-polysaccharide structure | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a Lipopolysaccharide | |
| 650 | 4 | |a O-antigen | |
| 650 | 4 | |a O-antigen gene cluster | |
| 650 | 4 | |a O-polysaccharide structure | |
| 650 | 4 | |a O-specific polysaccharide | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a O Antigens |2 NLM | |
| 650 | 7 | |a Polysaccharides, Bacterial |2 NLM | |
| 700 | 1 | |a Chen, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Senchenkova, Sofya N |e verfasserin |4 aut | |
| 700 | 1 | |a Filatov, Andrei V |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jingjie |e verfasserin |4 aut | |
| 700 | 1 | |a Shashkov, Alexander S |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Knirel, Yuriy A |e verfasserin |4 aut | |
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