Immunohistochemical Localization of Fibrinogen C Domain Containing 1 on Epithelial and Mucosal Surfaces in Human Tissues
Fibrinogen C domain containing 1 (FIBCD1) is a transmembrane receptor that binds chitin and other acetylated compounds with high affinity. FIBCD1 has previously been shown to be present in the epithelium of the gastrointestinal tract. In the present study, we performed a detailed analysis of normally structured human tissues for the expression of FIBCD1 by quantitative PCR and immunohistochemistry. We find that FIBCD1 is expressed in epithelial cells derived from all three germ layers. Endodermal-derived epithelial cells throughout the gastrointestinal tract and the respiratory system showed high expression of FIBCD1 and also mesodermal-derived cells in the genitourinary system and ectodermal-derived epidermis and sebaceous glands cells expressed FIBCD1. In some columnar epithelial cells, for example, in the salivary gland and gall bladder, the FIBCD1 expression was clearly polarized with strong apical reaction, while other columnar cells, for example, in small and large intestine and in bronchi, the staining was equally strong apically and basolaterally. In keratinocytes in skin, tongue, and oral cavity, the FIBCD1 staining was granular. This expression pattern together with the known binding properties supports that FIBCD1 plays a role in innate immunity in the skin and at mucosal surfaces.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:66 |
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| Enthalten in: |
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 66(2018), 2 vom: 02. Feb., Seite 85-97 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
von Huth, Sebastian [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.07.2019 Date Revised 30.07.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1369/0022155417743694 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM278856551 |
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| 100 | 1 | |a von Huth, Sebastian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Immunohistochemical Localization of Fibrinogen C Domain Containing 1 on Epithelial and Mucosal Surfaces in Human Tissues |
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| 500 | |a Date Revised 30.07.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Fibrinogen C domain containing 1 (FIBCD1) is a transmembrane receptor that binds chitin and other acetylated compounds with high affinity. FIBCD1 has previously been shown to be present in the epithelium of the gastrointestinal tract. In the present study, we performed a detailed analysis of normally structured human tissues for the expression of FIBCD1 by quantitative PCR and immunohistochemistry. We find that FIBCD1 is expressed in epithelial cells derived from all three germ layers. Endodermal-derived epithelial cells throughout the gastrointestinal tract and the respiratory system showed high expression of FIBCD1 and also mesodermal-derived cells in the genitourinary system and ectodermal-derived epidermis and sebaceous glands cells expressed FIBCD1. In some columnar epithelial cells, for example, in the salivary gland and gall bladder, the FIBCD1 expression was clearly polarized with strong apical reaction, while other columnar cells, for example, in small and large intestine and in bronchi, the staining was equally strong apically and basolaterally. In keratinocytes in skin, tongue, and oral cavity, the FIBCD1 staining was granular. This expression pattern together with the known binding properties supports that FIBCD1 plays a role in innate immunity in the skin and at mucosal surfaces | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a FIBCD1 | |
| 650 | 4 | |a chitin | |
| 650 | 4 | |a digestive system | |
| 650 | 4 | |a fibrinogen-related domain | |
| 650 | 4 | |a immunohistochemistry | |
| 650 | 4 | |a innate immunity | |
| 650 | 4 | |a mucosal immunity | |
| 650 | 4 | |a pattern recognition | |
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| 650 | 4 | |a respiratory system | |
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| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Receptors, Cell Surface |2 NLM | |
| 700 | 1 | |a Moeller, Jesper B |e verfasserin |4 aut | |
| 700 | 1 | |a Schlosser, Anders |e verfasserin |4 aut | |
| 700 | 1 | |a Marcussen, Niels |e verfasserin |4 aut | |
| 700 | 1 | |a Nielsen, Ole |e verfasserin |4 aut | |
| 700 | 1 | |a Nielsen, Vicki |e verfasserin |4 aut | |
| 700 | 1 | |a Sorensen, Grith L |e verfasserin |4 aut | |
| 700 | 1 | |a Holmskov, Uffe |e verfasserin |4 aut | |
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