Compositional Monosaccharide Analysis of Morus nigra Linn by HPLC and HPCE Quantitative Determination and Comparison of Polysaccharide from Morus nigra Linn by HPCE and HPLC
BACKGROUND: Morus nigra Linn is not only treated as health food but also medicine in Chinese history.
OBJECTIVE: Here, we have extracted and separated the heteroglycan to monosaccharides of the dry fruits.
METHOD: After heteroglycan being hydrolysised, then were we derivatived the monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP), and then subsequently HPCE and HPLC were used to separate. HPCE, which held an uncoated capillary (d=75μm) and detected by PDA at 245 nm with borate buffer, the voltage was set at 15 kV and capillary temperature was 25°C. There is Yilite column (Hypersil BDS C18 5μm, 4.6mm×250mm) with HPLC and detected by UV at 245 nm and capillary temperature 25°C.The High Performance capillary electrophoresis method and High Performance Liquid Chromatog-raphy method were compared on monosaccharide composition and quantitative determination of polysac-charide from the black mulberry.
RESULTS: Through several indicators including standard curve, precision, reproducibility, stability and recovery rate, the result of the two methods is basically consistent while they have complementary ad-vantages.
CONCLUSION: Both methods are suitable for the determination of the black mulberry polysaccharide, but each has its own merits.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
|---|---|
| Enthalten in: |
Current pharmaceutical analysis - 13(2017), 5 vom: 06. Okt., Seite 433-437 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ma, Xiao-Li [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Boric acid |
|---|
| Anmerkungen: |
Date Revised 27.03.2024 published: Print Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.2174/1573412913666170330150807 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM278783864 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM278783864 | ||
| 003 | DE-627 | ||
| 005 | 20240327233623.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.2174/1573412913666170330150807 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1350.xml |
| 035 | |a (DE-627)NLM278783864 | ||
| 035 | |a (NLM)29213223 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ma, Xiao-Li |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Compositional Monosaccharide Analysis of Morus nigra Linn by HPLC and HPCE Quantitative Determination and Comparison of Polysaccharide from Morus nigra Linn by HPCE and HPLC |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 27.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: Morus nigra Linn is not only treated as health food but also medicine in Chinese history | ||
| 520 | |a OBJECTIVE: Here, we have extracted and separated the heteroglycan to monosaccharides of the dry fruits | ||
| 520 | |a METHOD: After heteroglycan being hydrolysised, then were we derivatived the monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP), and then subsequently HPCE and HPLC were used to separate. HPCE, which held an uncoated capillary (d=75μm) and detected by PDA at 245 nm with borate buffer, the voltage was set at 15 kV and capillary temperature was 25°C. There is Yilite column (Hypersil BDS C18 5μm, 4.6mm×250mm) with HPLC and detected by UV at 245 nm and capillary temperature 25°C.The High Performance capillary electrophoresis method and High Performance Liquid Chromatog-raphy method were compared on monosaccharide composition and quantitative determination of polysac-charide from the black mulberry | ||
| 520 | |a RESULTS: Through several indicators including standard curve, precision, reproducibility, stability and recovery rate, the result of the two methods is basically consistent while they have complementary ad-vantages | ||
| 520 | |a CONCLUSION: Both methods are suitable for the determination of the black mulberry polysaccharide, but each has its own merits | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HPCE | |
| 650 | 4 | |a HPLC | |
| 650 | 4 | |a Morus nigra Linn | |
| 650 | 4 | |a boric acid | |
| 650 | 4 | |a linearity | |
| 650 | 4 | |a polysaccharide | |
| 700 | 1 | |a Song, Feng Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Huan, Xie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Song Ya |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Current pharmaceutical analysis |d 2008 |g 13(2017), 5 vom: 06. Okt., Seite 433-437 |w (DE-627)NLM240857917 |x 1573-4129 |7 nnns |
| 773 | 1 | 8 | |g volume:13 |g year:2017 |g number:5 |g day:06 |g month:10 |g pages:433-437 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.2174/1573412913666170330150807 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 13 |j 2017 |e 5 |b 06 |c 10 |h 433-437 | ||