Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease
© American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com.
OBJECTIVES: Investigate subtle fibrosis similarities and differences in adult and pediatric nonalcoholic fatty liver disease (NAFLD) using second harmonic generation (SHG).
METHODS: SHG/two-photon excitation fluorescence imaging quantified 100 collagen parameters and determined qFibrosis values by using the nonalcoholic steatohepatitis (NASH) Clinical Research Network (CRN) scoring system in 62 adult and 36 pediatric NAFLD liver specimens.
RESULTS: Six distinct parameters identified differences among the NASH CRN stages with high accuracy (area under the curve, 0835-0.982 vs 0.885-0.981, adult and pediatric). All portal region parameters showed similar changes across early stages 0, 1C, and 2, in both groups. Parameter values decreased in adults with progression from stage 1A/B to 2 in the central vein region. In children, aggregated collagen parameters decreased, but nearly all distributed collagen parameters increased from stage 1A/B to 2.
CONCLUSIONS: SHG analysis accurately reproduces NASH CRN staging in NAFLD, as well as reveals differences and similarities between adult and pediatric collagen deposition not captured by currently available quantitative methods.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:148 |
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| Enthalten in: |
American journal of clinical pathology - 148(2017), 6 vom: 20. Nov., Seite 502-512 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Feng [VerfasserIn] |
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| Links: |
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| Themen: |
Adult |
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| Anmerkungen: |
Date Completed 07.12.2017 Date Revised 16.03.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/ajcp/aqx104 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM278316328 |
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| 245 | 1 | 0 | |a Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease |
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| 500 | |a Date Revised 16.03.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com | ||
| 520 | |a OBJECTIVES: Investigate subtle fibrosis similarities and differences in adult and pediatric nonalcoholic fatty liver disease (NAFLD) using second harmonic generation (SHG) | ||
| 520 | |a METHODS: SHG/two-photon excitation fluorescence imaging quantified 100 collagen parameters and determined qFibrosis values by using the nonalcoholic steatohepatitis (NASH) Clinical Research Network (CRN) scoring system in 62 adult and 36 pediatric NAFLD liver specimens | ||
| 520 | |a RESULTS: Six distinct parameters identified differences among the NASH CRN stages with high accuracy (area under the curve, 0835-0.982 vs 0.885-0.981, adult and pediatric). All portal region parameters showed similar changes across early stages 0, 1C, and 2, in both groups. Parameter values decreased in adults with progression from stage 1A/B to 2 in the central vein region. In children, aggregated collagen parameters decreased, but nearly all distributed collagen parameters increased from stage 1A/B to 2 | ||
| 520 | |a CONCLUSIONS: SHG analysis accurately reproduces NASH CRN staging in NAFLD, as well as reveals differences and similarities between adult and pediatric collagen deposition not captured by currently available quantitative methods | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Adult | |
| 650 | 4 | |a Automated quantitative analysis | |
| 650 | 4 | |a Difference | |
| 650 | 4 | |a Liver fibrosis | |
| 650 | 4 | |a Nonalcoholic fatty liver disease | |
| 650 | 4 | |a Pediatric | |
| 650 | 4 | |a Second harmonic generation/two-photon excitation fluorescence | |
| 700 | 1 | |a Zhao, Jing-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Rao, Hui-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Wei-Miao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Theise, Neil D |e verfasserin |4 aut | |
| 700 | 1 | |a Wee, Aileen |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Lai |e verfasserin |4 aut | |
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