Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium
Transport of membrane and cytosolic proteins in primary cilia is thought to depend on intraflagellar transport (IFT) and diffusion. However, the relative contribution and spatial routes of each transport mechanism are largely unknown. Although challenging to decipher, the details of these routes are essential for our understanding of protein transport in primary cilia, a critically affected process in many genetic diseases. By using a high-speed virtual 3D super-resolution microscopy, we have mapped the 3D spatial locations of transport routes for various cytosolic proteins in the 250-nm-wide shaft of live primary cilia with a spatiotemporal resolution of 2 ms and <16 nm. Our data reveal two spatially distinguishable transport routes for cytosolic proteins: an IFT-dependent path along the axoneme, and a passive-diffusion route in the axonemal lumen that escaped previous studies. While all cytosolic proteins tested primarily utilize the IFT path in the anterograde direction, differences are observed in the retrograde direction where IFT20 only utilizes IFT, and approximately half of KIF17 and one third of α-tubulin utilizes diffusion besides IFT.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
Scientific reports - 7(2017), 1 vom: 17. Nov., Seite 15793 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Luo, Wangxi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
147336-22-9 |
|---|
| Anmerkungen: |
Date Completed 09.07.2019 Date Revised 04.12.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1038/s41598-017-16103-z |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM278168736 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM278168736 | ||
| 003 | DE-627 | ||
| 005 | 20231226194519.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41598-017-16103-z |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0927.xml |
| 035 | |a (DE-627)NLM278168736 | ||
| 035 | |a (NLM)29150645 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Luo, Wangxi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.07.2019 | ||
| 500 | |a Date Revised 04.12.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Transport of membrane and cytosolic proteins in primary cilia is thought to depend on intraflagellar transport (IFT) and diffusion. However, the relative contribution and spatial routes of each transport mechanism are largely unknown. Although challenging to decipher, the details of these routes are essential for our understanding of protein transport in primary cilia, a critically affected process in many genetic diseases. By using a high-speed virtual 3D super-resolution microscopy, we have mapped the 3D spatial locations of transport routes for various cytosolic proteins in the 250-nm-wide shaft of live primary cilia with a spatiotemporal resolution of 2 ms and <16 nm. Our data reveal two spatially distinguishable transport routes for cytosolic proteins: an IFT-dependent path along the axoneme, and a passive-diffusion route in the axonemal lumen that escaped previous studies. While all cytosolic proteins tested primarily utilize the IFT path in the anterograde direction, differences are observed in the retrograde direction where IFT20 only utilizes IFT, and approximately half of KIF17 and one third of α-tubulin utilizes diffusion besides IFT | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Ift20 protein, mouse |2 NLM | |
| 650 | 7 | |a KIF17 protein, mouse |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a Tubulin |2 NLM | |
| 650 | 7 | |a Green Fluorescent Proteins |2 NLM | |
| 650 | 7 | |a 147336-22-9 |2 NLM | |
| 650 | 7 | |a Kinesins |2 NLM | |
| 650 | 7 | |a EC 3.6.4.4 |2 NLM | |
| 700 | 1 | |a Ruba, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Takao, Daisuke |e verfasserin |4 aut | |
| 700 | 1 | |a Zweifel, Ludovit P |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Roderick Y H |e verfasserin |4 aut | |
| 700 | 1 | |a Verhey, Kristen J |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Weidong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d 2011 |g 7(2017), 1 vom: 17. Nov., Seite 15793 |w (DE-627)NLM215703936 |x 2045-2322 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2017 |g number:1 |g day:17 |g month:11 |g pages:15793 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41598-017-16103-z |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2017 |e 1 |b 17 |c 11 |h 15793 | ||