Association of insertion-deletions polymorphisms with colorectal cancer risk and clinical features
AIM: To investigate the association between 16 insertion-deletions (INDEL) polymorphisms, colorectal cancer (CRC) risk and clinical features in an admixed population.
METHODS: One hundred and forty patients with CRC and 140 cancer-free subjects were examined. Genomic DNA was extracted from peripheral blood samples. Polymorphisms and genomic ancestry distribution were assayed by Multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on GeneMapper ID v3.2. Clinicopathological data were obtained by consulting the patients' clinical charts, intra-operative documentation, and pathology scoring.
RESULTS: Logistic regression analysis showed that polymorphism variations in IL4 gene was associated with increased CRC risk, while TYMS and UCP2 genes were associated with decreased risk. Reference to anatomical localization of tumor Del allele of NFKB1 and CASP8 were associated with more colon related incidents than rectosigmoid. In relation to the INDEL association with tumor node metastasis (TNM) stage risk, the Ins alleles of ACE, HLAG and TP53 (6 bp INDEL) were associated with higher TNM stage. Furthermore, regarding INDEL association with relapse risk, the Ins alleles of ACE, HLAG, and UGT1A1 were associated with early relapse risk, as well as the Del allele of TYMS. Regarding INDEL association with death risk before 10 years, the Ins allele of SGSM3 and UGT1A1 were associated with death risk.
CONCLUSION: The INDEL variations in ACE, UCP2, TYMS, IL4, NFKB1, CASP8, TP53, HLAG, UGT1A1, and SGSM3 were associated with CRC risk and clinical features in an admixed population. These data suggest that this cancer panel might be useful as a complementary tool for better clinical management, and more studies need to be conducted to confirm these findings.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
|---|---|
| Enthalten in: |
World journal of gastroenterology - 23(2017), 37 vom: 07. Okt., Seite 6854-6867 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Marques, Diego [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Admixed population |
|---|
| Anmerkungen: |
Date Completed 15.06.2018 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.3748/wjg.v23.i37.6854 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM277526841 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM277526841 | ||
| 003 | DE-627 | ||
| 005 | 20231225014420.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3748/wjg.v23.i37.6854 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0925.xml |
| 035 | |a (DE-627)NLM277526841 | ||
| 035 | |a (NLM)29085228 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Marques, Diego |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Association of insertion-deletions polymorphisms with colorectal cancer risk and clinical features |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 15.06.2018 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a AIM: To investigate the association between 16 insertion-deletions (INDEL) polymorphisms, colorectal cancer (CRC) risk and clinical features in an admixed population | ||
| 520 | |a METHODS: One hundred and forty patients with CRC and 140 cancer-free subjects were examined. Genomic DNA was extracted from peripheral blood samples. Polymorphisms and genomic ancestry distribution were assayed by Multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on GeneMapper ID v3.2. Clinicopathological data were obtained by consulting the patients' clinical charts, intra-operative documentation, and pathology scoring | ||
| 520 | |a RESULTS: Logistic regression analysis showed that polymorphism variations in IL4 gene was associated with increased CRC risk, while TYMS and UCP2 genes were associated with decreased risk. Reference to anatomical localization of tumor Del allele of NFKB1 and CASP8 were associated with more colon related incidents than rectosigmoid. In relation to the INDEL association with tumor node metastasis (TNM) stage risk, the Ins alleles of ACE, HLAG and TP53 (6 bp INDEL) were associated with higher TNM stage. Furthermore, regarding INDEL association with relapse risk, the Ins alleles of ACE, HLAG, and UGT1A1 were associated with early relapse risk, as well as the Del allele of TYMS. Regarding INDEL association with death risk before 10 years, the Ins allele of SGSM3 and UGT1A1 were associated with death risk | ||
| 520 | |a CONCLUSION: The INDEL variations in ACE, UCP2, TYMS, IL4, NFKB1, CASP8, TP53, HLAG, UGT1A1, and SGSM3 were associated with CRC risk and clinical features in an admixed population. These data suggest that this cancer panel might be useful as a complementary tool for better clinical management, and more studies need to be conducted to confirm these findings | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Admixed population | |
| 650 | 4 | |a Clinical features | |
| 650 | 4 | |a Colorectal cancer | |
| 650 | 4 | |a Diagnostic | |
| 650 | 4 | |a Ins-del polymorphism | |
| 650 | 4 | |a Potential biomarker | |
| 650 | 4 | |a Prognostic | |
| 650 | 4 | |a Risk stratification | |
| 700 | 1 | |a Ferreira-Costa, Layse Raynara |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira-Costa, Lorenna Larissa |e verfasserin |4 aut | |
| 700 | 1 | |a Correa, Romualdo da Silva |e verfasserin |4 aut | |
| 700 | 1 | |a Borges, Aline Maciel Pinheiro |e verfasserin |4 aut | |
| 700 | 1 | |a Ito, Fernanda Ribeiro |e verfasserin |4 aut | |
| 700 | 1 | |a Ramos, Carlos Cesar de Oliveira |e verfasserin |4 aut | |
| 700 | 1 | |a Bortolin, Raul Hernandes |e verfasserin |4 aut | |
| 700 | 1 | |a Luchessi, André Ducati |e verfasserin |4 aut | |
| 700 | 1 | |a Ribeiro-Dos-Santos, Ândrea |e verfasserin |4 aut | |
| 700 | 1 | |a Santos, Sidney |e verfasserin |4 aut | |
| 700 | 1 | |a Silbiger, Vivian Nogueira |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t World journal of gastroenterology |d 1997 |g 23(2017), 37 vom: 07. Okt., Seite 6854-6867 |w (DE-627)NLM117065250 |x 2219-2840 |7 nnns |
| 773 | 1 | 8 | |g volume:23 |g year:2017 |g number:37 |g day:07 |g month:10 |g pages:6854-6867 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3748/wjg.v23.i37.6854 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 23 |j 2017 |e 37 |b 07 |c 10 |h 6854-6867 | ||