Better platelet function, less fibrinolysis and less hemolysis in re-transfused residual pump blood with the Ringer's chase technique - a randomized pilot study
INTRODUCTION: Residual pump blood from the cardiopulmonary bypass (CPB) circuit is often collected into an infusion bag (IB) and re-transfused. An alternative is to chase the residual blood into the circulation through the arterial cannula with Ringer's acetate. Our aim was to assess possible differences in hemostatic blood quality between these two techniques.
METHODS: Forty adult patients undergoing elective coronary artery bypass graft surgery with CPB were randomized to receive the residual pump blood by either an IB or through the Ringer's chase (RC) technique. Platelet activation and function (impedance aggregometry), coagulation and hemolysis variables were assessed in the re-transfused blood and in the patients before, during and after surgery. Results are presented as median (25-75 quartiles).
RESULTS: Total hemoglobin and platelet levels in the re-transfused blood were comparable with the two methods, as were soluble platelet activation markers P-selectin and soluble glycoprotein VI (GPVI). Platelet aggregation (U) in the IB blood was significantly lower compared to the RC blood, with the agonists adenosine diphosphate (ADP) 24 (10-32) vs 46 (33-65), p<0.01, thrombin receptor activating peptide (TRAP) 50 (29-73) vs 69 (51-92), p=0.04 and collagen 24 (17-28) vs 34 (26-59), p<0.01. The IB blood had higher amounts of free hemoglobin (mg/L) (1086 (891-1717) vs 591(517-646), p<0.01) and D-dimer 0.60 (0.33-0.98) vs 0.3 (0.3-0.48), p<0.01. Other coagulation variables showed no difference between the groups.
CONCLUSIONS: The handling of blood after CPB increases hemolysis, impairs platelet function and activates coagulation and fibrinolysis. The RC technique preserved the blood better than the commonly used IB technique.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
|---|---|
| Enthalten in: |
Perfusion - 33(2018), 3 vom: 10. Apr., Seite 185-193 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Olsson, Anki [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cardiopulmonary bypass |
|---|
| Anmerkungen: |
Date Completed 01.10.2018 Date Revised 01.10.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1177/0267659117733891 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM276208471 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM276208471 | ||
| 003 | DE-627 | ||
| 005 | 20231225011501.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1177/0267659117733891 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0920.xml |
| 035 | |a (DE-627)NLM276208471 | ||
| 035 | |a (NLM)28950757 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Olsson, Anki |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Better platelet function, less fibrinolysis and less hemolysis in re-transfused residual pump blood with the Ringer's chase technique - a randomized pilot study |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 01.10.2018 | ||
| 500 | |a Date Revised 01.10.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: Residual pump blood from the cardiopulmonary bypass (CPB) circuit is often collected into an infusion bag (IB) and re-transfused. An alternative is to chase the residual blood into the circulation through the arterial cannula with Ringer's acetate. Our aim was to assess possible differences in hemostatic blood quality between these two techniques | ||
| 520 | |a METHODS: Forty adult patients undergoing elective coronary artery bypass graft surgery with CPB were randomized to receive the residual pump blood by either an IB or through the Ringer's chase (RC) technique. Platelet activation and function (impedance aggregometry), coagulation and hemolysis variables were assessed in the re-transfused blood and in the patients before, during and after surgery. Results are presented as median (25-75 quartiles) | ||
| 520 | |a RESULTS: Total hemoglobin and platelet levels in the re-transfused blood were comparable with the two methods, as were soluble platelet activation markers P-selectin and soluble glycoprotein VI (GPVI). Platelet aggregation (U) in the IB blood was significantly lower compared to the RC blood, with the agonists adenosine diphosphate (ADP) 24 (10-32) vs 46 (33-65), p<0.01, thrombin receptor activating peptide (TRAP) 50 (29-73) vs 69 (51-92), p=0.04 and collagen 24 (17-28) vs 34 (26-59), p<0.01. The IB blood had higher amounts of free hemoglobin (mg/L) (1086 (891-1717) vs 591(517-646), p<0.01) and D-dimer 0.60 (0.33-0.98) vs 0.3 (0.3-0.48), p<0.01. Other coagulation variables showed no difference between the groups | ||
| 520 | |a CONCLUSIONS: The handling of blood after CPB increases hemolysis, impairs platelet function and activates coagulation and fibrinolysis. The RC technique preserved the blood better than the commonly used IB technique | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a cardiopulmonary bypass | |
| 650 | 4 | |a hemostasis | |
| 650 | 4 | |a methods | |
| 650 | 4 | |a platelet function tests | |
| 650 | 7 | |a Isotonic Solutions |2 NLM | |
| 650 | 7 | |a Ringer's acetate |2 NLM | |
| 700 | 1 | |a Alfredsson, Joakim |e verfasserin |4 aut | |
| 700 | 1 | |a Ramström, Sofia |e verfasserin |4 aut | |
| 700 | 1 | |a Svedjeholm, Rolf |e verfasserin |4 aut | |
| 700 | 1 | |a Kenny, Dermot |e verfasserin |4 aut | |
| 700 | 1 | |a Håkansson, Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Berglund, Johan Sanmartin |e verfasserin |4 aut | |
| 700 | 1 | |a Berg, Sören |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Perfusion |d 1990 |g 33(2018), 3 vom: 10. Apr., Seite 185-193 |w (DE-627)NLM074653768 |x 1477-111X |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2018 |g number:3 |g day:10 |g month:04 |g pages:185-193 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1177/0267659117733891 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 33 |j 2018 |e 3 |b 10 |c 04 |h 185-193 | ||