Revalorisation of rapeseed pomace extracts : An in vitro study into its anti-oxidant and DNA protective properties
Copyright © 2017 Elsevier Ltd. All rights reserved..
Rapeseed pomace (RSP) is a waste product obtained after edible oil production from Brassica napus. Analysis of ubiquitous secondary metabolites in RSP samples (two breeds, harvested in 2012/2014 respectively from North East of Scotland) and their ethanol/water (95:5) Soxhlet extracts were carried out. Soxhlet extraction of the RSP (petroleum ether followed by 95% ethanol) gave a solid extract. LC-MS/MS data of the extracts revealed several secondary metabolites, with Sinapic acid being the most abundant. Strong antioxidant activities of the Soxhlet extracts were confirmed from the results obtained in the FRAP, DPPH and ORAC assays. Furthermore, for the very first time, RSP extracts (13.9µg/ml) provided complete DNA protection, from oxidative stress induced by AAPH (3.5mM). Therefore the strong antioxidant and DNA protecting properties demonstrated by the RSP extracts in this study warrants further investigation for their revalorisation and potential use as reliable source of antioxidants in different food applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:239 |
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| Enthalten in: |
Food chemistry - 239(2018) vom: 15. Jan., Seite 323-332 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pohl, Franziska [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.11.2017 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2017.06.129 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM275455777 |
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| 245 | 1 | 0 | |a Revalorisation of rapeseed pomace extracts |b An in vitro study into its anti-oxidant and DNA protective properties |
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| 500 | |a Date Completed 20.11.2017 | ||
| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2017 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Rapeseed pomace (RSP) is a waste product obtained after edible oil production from Brassica napus. Analysis of ubiquitous secondary metabolites in RSP samples (two breeds, harvested in 2012/2014 respectively from North East of Scotland) and their ethanol/water (95:5) Soxhlet extracts were carried out. Soxhlet extraction of the RSP (petroleum ether followed by 95% ethanol) gave a solid extract. LC-MS/MS data of the extracts revealed several secondary metabolites, with Sinapic acid being the most abundant. Strong antioxidant activities of the Soxhlet extracts were confirmed from the results obtained in the FRAP, DPPH and ORAC assays. Furthermore, for the very first time, RSP extracts (13.9µg/ml) provided complete DNA protection, from oxidative stress induced by AAPH (3.5mM). Therefore the strong antioxidant and DNA protecting properties demonstrated by the RSP extracts in this study warrants further investigation for their revalorisation and potential use as reliable source of antioxidants in different food applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ferric iron reducing antioxidant power (FRAP) | |
| 650 | 4 | |a Oxygen-radical absorbance capacity assay (ORAC) | |
| 650 | 4 | |a Phenolics | |
| 650 | 4 | |a Radical scavenging activity (DPPH) | |
| 650 | 4 | |a Rapeseed pomace | |
| 650 | 4 | |a Reducing capacity (FC) | |
| 650 | 4 | |a Soxhlet extraction | |
| 650 | 4 | |a pBR322 plasmid DNA | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a DNA, Plant |2 NLM | |
| 650 | 7 | |a Oxidants |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 700 | 1 | |a Goua, Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Bermano, Giovanna |e verfasserin |4 aut | |
| 700 | 1 | |a Russell, Wendy R |e verfasserin |4 aut | |
| 700 | 1 | |a Scobbie, Lorraine |e verfasserin |4 aut | |
| 700 | 1 | |a Maciel, Patrícia |e verfasserin |4 aut | |
| 700 | 1 | |a Kong Thoo Lin, Paul |e verfasserin |4 aut | |
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