Cyclooxygenase-2 contributes to oxidopamine-mediated neuronal inflammation and injury via the prostaglandin E2 receptor EP2 subtype
Cyclooxygenase-2 (COX-2) triggers pro-inflammatory processes that can aggravate neuronal degeneration and functional impairments in many neurological conditions, mainly via producing prostaglandin E2 (PGE2) that activates four membrane receptors, EP1-EP4. However, which EP receptor is the culprit of COX-2/PGE2-mediated neuronal inflammation and degeneration remains largely unclear and presumably depends on the insult types and responding components. Herein, we demonstrated that COX-2 was induced and showed nuclear translocation in two neuronal cell lines - mouse Neuro-2a and human SH-SY5Y - after treatment with neurotoxin 6-hydroxydopamine (6-OHDA), leading to the biosynthesis of PGE2 and upregulation of pro-inflammatory cytokine interleukin-1β. Inhibiting COX-2 or microsomal prostaglandin E synthase-1 suppressed the 6-OHDA-triggered PGE2 production in these cells. Treatment with PGE2 or EP2 selective agonist butaprost, but not EP4 agonist CAY10598, increased cAMP response in both cell lines. PGE2-initiated cAMP production in these cells was blocked by our recently developed novel selective EP2 antagonists - TG4-155 and TG6-10-1, but not by EP4 selective antagonist GW627368X. The 6-OHDA-promoted cytotoxicity was largely blocked by TG4-155, TG6-10-1 or COX-2 selective inhibitor celecoxib, but not by GW627368X. Our results suggest that PGE2 receptor EP2 is a key mediator of COX-2 activity-initiated cAMP signaling in Neuro-2a and SH-SY5Y cells following 6-OHDA treatment, and contributes to oxidopamine-mediated neurotoxicity.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2017 |
---|---|
Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
---|---|
Enthalten in: |
Scientific reports - 7(2017), 1 vom: 25. Aug., Seite 9459 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Kang, Xu [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 08.04.2019 Date Revised 08.04.2019 published: Electronic Citation Status MEDLINE |
---|
doi: |
10.1038/s41598-017-09528-z |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM275154270 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM275154270 | ||
003 | DE-627 | ||
005 | 20231226194225.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2017 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1038/s41598-017-09528-z |2 doi | |
028 | 5 | 2 | |a pubmed24n0917.xml |
035 | |a (DE-627)NLM275154270 | ||
035 | |a (NLM)28842681 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Kang, Xu |e verfasserin |4 aut | |
245 | 1 | 0 | |a Cyclooxygenase-2 contributes to oxidopamine-mediated neuronal inflammation and injury via the prostaglandin E2 receptor EP2 subtype |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 08.04.2019 | ||
500 | |a Date Revised 08.04.2019 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Cyclooxygenase-2 (COX-2) triggers pro-inflammatory processes that can aggravate neuronal degeneration and functional impairments in many neurological conditions, mainly via producing prostaglandin E2 (PGE2) that activates four membrane receptors, EP1-EP4. However, which EP receptor is the culprit of COX-2/PGE2-mediated neuronal inflammation and degeneration remains largely unclear and presumably depends on the insult types and responding components. Herein, we demonstrated that COX-2 was induced and showed nuclear translocation in two neuronal cell lines - mouse Neuro-2a and human SH-SY5Y - after treatment with neurotoxin 6-hydroxydopamine (6-OHDA), leading to the biosynthesis of PGE2 and upregulation of pro-inflammatory cytokine interleukin-1β. Inhibiting COX-2 or microsomal prostaglandin E synthase-1 suppressed the 6-OHDA-triggered PGE2 production in these cells. Treatment with PGE2 or EP2 selective agonist butaprost, but not EP4 agonist CAY10598, increased cAMP response in both cell lines. PGE2-initiated cAMP production in these cells was blocked by our recently developed novel selective EP2 antagonists - TG4-155 and TG6-10-1, but not by EP4 selective antagonist GW627368X. The 6-OHDA-promoted cytotoxicity was largely blocked by TG4-155, TG6-10-1 or COX-2 selective inhibitor celecoxib, but not by GW627368X. Our results suggest that PGE2 receptor EP2 is a key mediator of COX-2 activity-initiated cAMP signaling in Neuro-2a and SH-SY5Y cells following 6-OHDA treatment, and contributes to oxidopamine-mediated neurotoxicity | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Cytokines |2 NLM | |
650 | 7 | |a Inflammation Mediators |2 NLM | |
650 | 7 | |a Receptors, Prostaglandin E, EP2 Subtype |2 NLM | |
650 | 7 | |a Oxidopamine |2 NLM | |
650 | 7 | |a 8HW4YBZ748 |2 NLM | |
650 | 7 | |a Cyclic AMP |2 NLM | |
650 | 7 | |a E0399OZS9N |2 NLM | |
650 | 7 | |a Cyclooxygenase 2 |2 NLM | |
650 | 7 | |a EC 1.14.99.1 |2 NLM | |
700 | 1 | |a Qiu, Jiange |e verfasserin |4 aut | |
700 | 1 | |a Li, Qianqian |e verfasserin |4 aut | |
700 | 1 | |a Bell, Katherine A |e verfasserin |4 aut | |
700 | 1 | |a Du, Yifeng |e verfasserin |4 aut | |
700 | 1 | |a Jung, Da Woon |e verfasserin |4 aut | |
700 | 1 | |a Lee, Jae Yeol |e verfasserin |4 aut | |
700 | 1 | |a Hao, Jiukuan |e verfasserin |4 aut | |
700 | 1 | |a Jiang, Jianxiong |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Scientific reports |d 2011 |g 7(2017), 1 vom: 25. Aug., Seite 9459 |w (DE-627)NLM215703936 |x 2045-2322 |7 nnns |
773 | 1 | 8 | |g volume:7 |g year:2017 |g number:1 |g day:25 |g month:08 |g pages:9459 |
856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41598-017-09528-z |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 7 |j 2017 |e 1 |b 25 |c 08 |h 9459 |