Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves
CONTEXT: Carica papaya Linn. (Caricaceae) leaf (CPL) juice has long been traditionally used in ethnomedicine for dengue fever.
OBJECTIVE: The study examines the effects of standardized CPL aqueous extract (SCPLE) on platelet count, extramedullary haematopoiesis (EMH), and immunomodulation in cyclophosphamide (CP)-induced animal model of thrombocytopenia.
MATERIALS AND METHODS: The extract was analyzed for myricetin, caffeic acid, trans-ferulic acid, and kaempferol using HPTLC for standardization followed by UPLC-qTOF/MS fingerprinting for metabolite signature. The effects of SCPLE (50 and 150 mg/kg p.o.) on proliferative response of platelet count and total leucocyte count (TLC) were observed up to 14 days in Wistar rat. However, delayed-type hypersensitivity (DTH), haemagglutination titre (HT), and in vivo carbon clearance were examined as immunomodulatory parameters in albino mice at 150 mg/kg p.o. against CP.
RESULTS: The quantitative HPTLC estimation of SCPLE showed the presence of myricetin, caffeic acid, trans-ferulic acid, and kaempferol up to 280.16 ± 5.99, 370.18 ± 6.27, 1110.86 ± 2.97, and 160.53 ± 2.48 (μg/g), respectively. Twenty-four metabolites were identified using UPLC-qTOF/MS. Oral administration of SCPLE (150 mg/kg) in thrombocytopenic rats exhibited significant (p < 0.01) increase in thrombocytes (1014.83 × 103 cells/mm3), DTH response (0.16 ± 0.004), and phagocytic index (63.15% increase) as compared to CP-induced thrombocytopenia group. Histopathological studies showed minimal fibrosis in spleen histology.
DISCUSSION AND CONCLUSIONS: Results suggest CPL can mediate the release of platelets providing the means for the treatment and prevention of dengue.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
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| Enthalten in: |
Pharmaceutical biology - 55(2017), 1 vom: 13. Dez., Seite 2043-2056 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Anjum, Varisha [VerfasserIn] |
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| Links: |
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| Themen: |
Dengue fever |
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| Anmerkungen: |
Date Completed 23.04.2018 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1080/13880209.2017.1346690 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM275093395 |
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| 245 | 1 | 0 | |a Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
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| 500 | |a Date Completed 23.04.2018 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a CONTEXT: Carica papaya Linn. (Caricaceae) leaf (CPL) juice has long been traditionally used in ethnomedicine for dengue fever | ||
| 520 | |a OBJECTIVE: The study examines the effects of standardized CPL aqueous extract (SCPLE) on platelet count, extramedullary haematopoiesis (EMH), and immunomodulation in cyclophosphamide (CP)-induced animal model of thrombocytopenia | ||
| 520 | |a MATERIALS AND METHODS: The extract was analyzed for myricetin, caffeic acid, trans-ferulic acid, and kaempferol using HPTLC for standardization followed by UPLC-qTOF/MS fingerprinting for metabolite signature. The effects of SCPLE (50 and 150 mg/kg p.o.) on proliferative response of platelet count and total leucocyte count (TLC) were observed up to 14 days in Wistar rat. However, delayed-type hypersensitivity (DTH), haemagglutination titre (HT), and in vivo carbon clearance were examined as immunomodulatory parameters in albino mice at 150 mg/kg p.o. against CP | ||
| 520 | |a RESULTS: The quantitative HPTLC estimation of SCPLE showed the presence of myricetin, caffeic acid, trans-ferulic acid, and kaempferol up to 280.16 ± 5.99, 370.18 ± 6.27, 1110.86 ± 2.97, and 160.53 ± 2.48 (μg/g), respectively. Twenty-four metabolites were identified using UPLC-qTOF/MS. Oral administration of SCPLE (150 mg/kg) in thrombocytopenic rats exhibited significant (p < 0.01) increase in thrombocytes (1014.83 × 103 cells/mm3), DTH response (0.16 ± 0.004), and phagocytic index (63.15% increase) as compared to CP-induced thrombocytopenia group. Histopathological studies showed minimal fibrosis in spleen histology | ||
| 520 | |a DISCUSSION AND CONCLUSIONS: Results suggest CPL can mediate the release of platelets providing the means for the treatment and prevention of dengue | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dengue fever | |
| 650 | 4 | |a UPLC-qTOF/MS | |
| 650 | 4 | |a immunomodulation | |
| 650 | 4 | |a thrombocytopenia | |
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| 650 | 7 | |a Plant Extracts |2 NLM | |
| 700 | 1 | |a Arora, Poonam |e verfasserin |4 aut | |
| 700 | 1 | |a Ansari, Shahid Husain |e verfasserin |4 aut | |
| 700 | 1 | |a Najmi, Abul Kalam |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmad, Sayeed |e verfasserin |4 aut | |
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