Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes
Arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. Chikungunya-affected areas often overlap with dengue-endemic areas. Concurrent dengue virus (DENV) and chikungunya virus (CHIKV) infections have been detected in travelers returning from regions of endemicity. CHIKV and DENV co-infected Aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosquitoes. We thus aimed to study the pathogen-pathogen interaction involved in these co-infections in DENV/CHIKV co-infected Aedes aegypti mosquitoes. In mono-infections, we detected CHIKV antigens as early as 4 days post-virus exposure in both the midgut (MG) and salivary gland (SG), whereas we detected DENV serotype 2 (DENV-2) antigens from day 5 post-virus exposure in MG and day 10 post-virus exposure in SG. Identical infection rates were observed for singly and co-infected mosquitoes, and facilitation of the replication of both viruses at various times post-viral exposure. We observed a higher replication for DENV-2 in SG of co-infected mosquitoes. We showed that mixed CHIKV and DENV infection facilitated viral replication in Ae. aegypti. The outcome of these mixed infections must be further studied to increase our understanding of pathogen-pathogen interactions in host cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
International journal of molecular sciences - 18(2017), 8 vom: 04. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Le Coupanec, Alain [VerfasserIn] |
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| Links: |
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| Themen: |
Aedes aegypti |
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| Anmerkungen: |
Date Completed 23.04.2018 Date Revised 13.11.2018 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms18081708 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM274512300 |
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| 245 | 1 | 0 | |a Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes |
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| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. Chikungunya-affected areas often overlap with dengue-endemic areas. Concurrent dengue virus (DENV) and chikungunya virus (CHIKV) infections have been detected in travelers returning from regions of endemicity. CHIKV and DENV co-infected Aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosquitoes. We thus aimed to study the pathogen-pathogen interaction involved in these co-infections in DENV/CHIKV co-infected Aedes aegypti mosquitoes. In mono-infections, we detected CHIKV antigens as early as 4 days post-virus exposure in both the midgut (MG) and salivary gland (SG), whereas we detected DENV serotype 2 (DENV-2) antigens from day 5 post-virus exposure in MG and day 10 post-virus exposure in SG. Identical infection rates were observed for singly and co-infected mosquitoes, and facilitation of the replication of both viruses at various times post-viral exposure. We observed a higher replication for DENV-2 in SG of co-infected mosquitoes. We showed that mixed CHIKV and DENV infection facilitated viral replication in Ae. aegypti. The outcome of these mixed infections must be further studied to increase our understanding of pathogen-pathogen interactions in host cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aedes aegypti | |
| 650 | 4 | |a arbovirus | |
| 650 | 4 | |a chikungunya | |
| 650 | 4 | |a co-infection | |
| 650 | 4 | |a dengue | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 700 | 1 | |a Tchankouo-Nguetcheu, Stéphane |e verfasserin |4 aut | |
| 700 | 1 | |a Roux, Pascal |e verfasserin |4 aut | |
| 700 | 1 | |a Khun, Huot |e verfasserin |4 aut | |
| 700 | 1 | |a Huerre, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a Morales-Vargas, Ronald |e verfasserin |4 aut | |
| 700 | 1 | |a Enguehard, Margot |e verfasserin |4 aut | |
| 700 | 1 | |a Lavillette, Dimitri |e verfasserin |4 aut | |
| 700 | 1 | |a Missé, Dorothée |e verfasserin |4 aut | |
| 700 | 1 | |a Choumet, Valérie |e verfasserin |4 aut | |
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