A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro
© 2017 International Society for Advancement of Cytometry..
A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine. © 2017 International Society for Advancement of Cytometry.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:91 |
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| Enthalten in: |
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 91(2017), 8 vom: 01. Aug., Seite 794-799 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tiryaki, Volkan Müjdat [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.04.2018 Date Revised 05.06.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/cyto.a.23183 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM274214229 |
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| 100 | 1 | |a Tiryaki, Volkan Müjdat |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro |
| 264 | 1 | |c 2017 | |
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| 500 | |a Date Completed 30.04.2018 | ||
| 500 | |a Date Revised 05.06.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2017 International Society for Advancement of Cytometry. | ||
| 520 | |a A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine. © 2017 International Society for Advancement of Cytometry | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a 3D shape descriptor | |
| 650 | 4 | |a cell morphology | |
| 650 | 4 | |a confocal laser scanning microscope | |
| 650 | 4 | |a immunoreactivity | |
| 650 | 4 | |a quantitative cell spreading | |
| 650 | 7 | |a Glial Fibrillary Acidic Protein |2 NLM | |
| 700 | 1 | |a Ayres, Virginia M |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmed, Ijaz |e verfasserin |4 aut | |
| 700 | 1 | |a Shreiber, David I |e verfasserin |4 aut | |
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