Lipid droplet size and location in human skeletal muscle fibers are associated with insulin sensitivity
Copyright © 2017 the American Physiological Society..
In skeletal muscle, an accumulation of lipid droplets (LDs) in the subsarcolemmal space is associated with insulin resistance, but the underlying mechanism is not clear. We aimed to investigate how the size, number, and location of LDs are associated with insulin sensitivity and muscle fiber types and are regulated by aerobic training and treatment with an erythropoiesis-stimulating agent (ESA) in healthy young untrained men. LD analyses were performed by quantitative transmission electron microscopy, and insulin sensitivity was assessed by a hyperinsulinemic-euglycemic clamp. At baseline, we found that only the diameter (and not the number) of individual subsarcolemmal LDs was negatively associated with insulin sensitivity (R2 = 0.20, P = 0.03, n = 29). Despite 34% (P = 0.004) fewer LDs, the diameter of individual subsarcolemmal LDs was 20% (P = 0.0004) larger in type 2 fibers than in type 1 fibers. Furthermore, aerobic training decreased the size of subsarcolemmal LDs in the type 2 fibers, and ESA treatment lowered the number of both intermyofibrillar and subsarcolemmal LDs in the type 1 fibers. In conclusion, the size of individual subsarcolemmal LDs may be involved in the mechanism by which LDs are associated with insulin resistance in skeletal muscle.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:313 |
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| Enthalten in: |
American journal of physiology. Endocrinology and metabolism - 313(2017), 6 vom: 01. Dez., Seite E721-E730 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nielsen, Joachim [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 19.12.2017 Date Revised 23.02.2018 published: Print-Electronic ClinicalTrials.gov: NCT01320449 Citation Status MEDLINE |
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| doi: |
10.1152/ajpendo.00062.2017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM274184699 |
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| 100 | 1 | |a Nielsen, Joachim |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lipid droplet size and location in human skeletal muscle fibers are associated with insulin sensitivity |
| 264 | 1 | |c 2017 | |
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| 500 | |a Date Completed 19.12.2017 | ||
| 500 | |a Date Revised 23.02.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ClinicalTrials.gov: NCT01320449 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2017 the American Physiological Society. | ||
| 520 | |a In skeletal muscle, an accumulation of lipid droplets (LDs) in the subsarcolemmal space is associated with insulin resistance, but the underlying mechanism is not clear. We aimed to investigate how the size, number, and location of LDs are associated with insulin sensitivity and muscle fiber types and are regulated by aerobic training and treatment with an erythropoiesis-stimulating agent (ESA) in healthy young untrained men. LD analyses were performed by quantitative transmission electron microscopy, and insulin sensitivity was assessed by a hyperinsulinemic-euglycemic clamp. At baseline, we found that only the diameter (and not the number) of individual subsarcolemmal LDs was negatively associated with insulin sensitivity (R2 = 0.20, P = 0.03, n = 29). Despite 34% (P = 0.004) fewer LDs, the diameter of individual subsarcolemmal LDs was 20% (P = 0.0004) larger in type 2 fibers than in type 1 fibers. Furthermore, aerobic training decreased the size of subsarcolemmal LDs in the type 2 fibers, and ESA treatment lowered the number of both intermyofibrillar and subsarcolemmal LDs in the type 1 fibers. In conclusion, the size of individual subsarcolemmal LDs may be involved in the mechanism by which LDs are associated with insulin resistance in skeletal muscle | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a aerobic training | |
| 650 | 4 | |a eryhropoietin | |
| 650 | 4 | |a insulin sensitivity | |
| 650 | 4 | |a intramuscular lipid droplets | |
| 650 | 4 | |a skeletal muscle fiber types | |
| 650 | 4 | |a transmission electron microscopy | |
| 700 | 1 | |a Christensen, Anders E |e verfasserin |4 aut | |
| 700 | 1 | |a Nellemann, Birgitte |e verfasserin |4 aut | |
| 700 | 1 | |a Christensen, Britt |e verfasserin |4 aut | |
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