Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:65 |
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| Enthalten in: |
Polish journal of microbiology - 65(2017), 4 vom: 02. Jan., Seite 479-483 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Figas, Agnieszka [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.12.2017 Date Revised 21.12.2017 published: Print Citation Status MEDLINE |
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| doi: |
10.5604/17331331.1227676 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM274101939 |
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| 520 | |a The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures | ||
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| 700 | 1 | |a Litwińska, Bogumiła |e verfasserin |4 aut | |
| 700 | 1 | |a Gut, Włodzimierz |e verfasserin |4 aut | |
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