SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion
SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion. [BMB Reports 2017; 50(8): 423-428].
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
|---|---|
| Enthalten in: |
BMB reports - 50(2017), 8 vom: 17. Aug., Seite 423-428 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Moon, Heegyum [VerfasserIn] |
|---|
| Themen: |
147153-65-9 |
|---|
| Anmerkungen: |
Date Completed 08.05.2018 Date Revised 09.06.2019 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM273880764 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM273880764 | ||
| 003 | DE-627 | ||
| 005 | 20231225002228.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0912.xml |
| 035 | |a (DE-627)NLM273880764 | ||
| 035 | |a (NLM)28712387 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Moon, Heegyum |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.05.2018 | ||
| 500 | |a Date Revised 09.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion. [BMB Reports 2017; 50(8): 423-428] | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a RNA Precursors |2 NLM | |
| 650 | 7 | |a RNA Splicing Factors |2 NLM | |
| 650 | 7 | |a SMN Complex Proteins |2 NLM | |
| 650 | 7 | |a SRSF2 protein, human |2 NLM | |
| 650 | 7 | |a 147153-65-9 |2 NLM | |
| 650 | 7 | |a Serine-Arginine Splicing Factors |2 NLM | |
| 650 | 7 | |a 170974-22-8 |2 NLM | |
| 700 | 1 | |a Cho, Sunghee |e verfasserin |4 aut | |
| 700 | 1 | |a Loh, Tiing Jen |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Ha Na |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yongchao |e verfasserin |4 aut | |
| 700 | 1 | |a Choi, Namjeong |e verfasserin |4 aut | |
| 700 | 1 | |a Oh, Jagyeong |e verfasserin |4 aut | |
| 700 | 1 | |a Ha, Jiyeon |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Sungchan |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Dong-Eun |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Michael B |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Xuexiu |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Haihong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BMB reports |d 2008 |g 50(2017), 8 vom: 17. Aug., Seite 423-428 |w (DE-627)NLM177900369 |x 1976-670X |7 nnns |
| 773 | 1 | 8 | |g volume:50 |g year:2017 |g number:8 |g day:17 |g month:08 |g pages:423-428 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 50 |j 2017 |e 8 |b 17 |c 08 |h 423-428 | ||