Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area
BACKGROUND: Sensitization to Pinales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens.
OBJECTIVES: To investigate the prevalence of allergy to different conifer pollens, describe the characteristics of patients with such allergy, and identify the involved allergens.
METHODS: Patients were recruited at five hospitals near Madrid. Extracts from conifer pollen were prepared and used in skin-prick testing. Wheal sizes were recorded, and serum samples obtained from patients with positive reactions to Cupressus arizonica and/or Pinus pinea. The specific immunoglobulin E value to C. arizonica and Cup a 1 was determined. Individual immunoblots for each patient and with a pool of sera were performed. Allergenic proteins were sequenced by using liquid chromatography-tandem mass spectrometry.
RESULTS: Of 499 individuals included in the study, 17 (14%) had positive skin-prick test results to some conifer pollen extracts. Sixty-four patients had positive results to C. arizonica (prevalence 12.8%) and 11 had positive results to P. pinea (2.2%). All the patients had respiratory symptoms (61.4% during the C. arizonica pollination period), and 62.9% had asthma. Approximately 86% of the patients had positive specific immunoglobulin E results to C. arizonica and 92.3% had positive results to Cup a 1. Fourteen different bands were recognized by immunoblot; the most frequent bands were those detected at 43, 18, 16, and 14 kDa. All sequenced proteins corresponded to Cup a 1.
CONCLUSION: Allergy to conifer pollen could be considered a relevant cause of respiratory allergy in central Spain. Asthma was more frequent than in other studies. We only identified Cup a 1 as involved in sensitization.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Allergy & rhinology (Providence, R.I.) - 7(2016), 4 vom: 01. Jan., Seite 200-206 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Domínguez-Ortega, Javier [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Revised 30.09.2020 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.2500/ar.2016.7.0183 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM273597361 |
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| 100 | 1 | |a Domínguez-Ortega, Javier |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
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| 500 | |a Date Revised 30.09.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: Sensitization to Pinales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens | ||
| 520 | |a OBJECTIVES: To investigate the prevalence of allergy to different conifer pollens, describe the characteristics of patients with such allergy, and identify the involved allergens | ||
| 520 | |a METHODS: Patients were recruited at five hospitals near Madrid. Extracts from conifer pollen were prepared and used in skin-prick testing. Wheal sizes were recorded, and serum samples obtained from patients with positive reactions to Cupressus arizonica and/or Pinus pinea. The specific immunoglobulin E value to C. arizonica and Cup a 1 was determined. Individual immunoblots for each patient and with a pool of sera were performed. Allergenic proteins were sequenced by using liquid chromatography-tandem mass spectrometry | ||
| 520 | |a RESULTS: Of 499 individuals included in the study, 17 (14%) had positive skin-prick test results to some conifer pollen extracts. Sixty-four patients had positive results to C. arizonica (prevalence 12.8%) and 11 had positive results to P. pinea (2.2%). All the patients had respiratory symptoms (61.4% during the C. arizonica pollination period), and 62.9% had asthma. Approximately 86% of the patients had positive specific immunoglobulin E results to C. arizonica and 92.3% had positive results to Cup a 1. Fourteen different bands were recognized by immunoblot; the most frequent bands were those detected at 43, 18, 16, and 14 kDa. All sequenced proteins corresponded to Cup a 1 | ||
| 520 | |a CONCLUSION: Allergy to conifer pollen could be considered a relevant cause of respiratory allergy in central Spain. Asthma was more frequent than in other studies. We only identified Cup a 1 as involved in sensitization | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a López-Matas, María Ángeles |e verfasserin |4 aut | |
| 700 | 1 | |a Alonso, María Dolores |e verfasserin |4 aut | |
| 700 | 1 | |a Feliú, Angélica |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Hornillos, Javier |e verfasserin |4 aut | |
| 700 | 1 | |a González, Emma |e verfasserin |4 aut | |
| 700 | 1 | |a Moya, Raquel |e verfasserin |4 aut | |
| 700 | 1 | |a Carnés, Jerónimo |e verfasserin |4 aut | |
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