An Elf2-like transcription factor acts as repressor of the mouse ecto-5'-nucleotidase gene expression in hepatic myofibroblasts
Hepatic fibrosis represents a pathological wound healing and tissue repair process triggered in response to chronic liver injury. A heterogeneous population of activated non-parenchymal liver cells, known as liver myofibroblasts, functions as the effector cells in hepatic fibrosis. Upon activation, liver myofibroblasts become fibrogenic, acquiring contractile properties and increasing collagen production capacity, while developing enhanced sensitivity to endogenous molecules and factors released in the local microenvironment. Hepatic extracellular adenosine is a bioactive small molecule, increasingly recognized as an important regulator of liver myofibroblast functions, and an important mediator in the pathogenesis of liver fibrosis overall. Remarkably, ecto-5'-nucleotidase/Nt5e/Cd73 enzyme, which accounts for the dominant adenosine-generating activity in the extracellular medium, is expressed by activated liver myofibroblasts. However, the molecular signals regulating Nt5e gene expression in liver myofibroblasts remain poorly understood. Here, we show that activated mouse liver myofibroblasts express Nt5e gene products and characterize the putative Nt5e minimal promoter in the mouse species. We describe the existence of an enhancer sequence upstream of the mouse Nt5e minimal promoter and establish that the mouse Nt5e minimal promoter transcriptional activity is negatively regulated by an Elf2-like Ets-related transcription factor in activated mouse liver myofibroblasts.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
|---|---|
| Enthalten in: |
Purinergic signalling - 13(2017), 4 vom: 01. Dez., Seite 417-428 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Fausther, Michel [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 27.07.2018 Date Revised 30.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s11302-017-9570-7 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM27344655X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM27344655X | ||
| 003 | DE-627 | ||
| 005 | 20240108140142.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11302-017-9570-7 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1243.xml |
| 035 | |a (DE-627)NLM27344655X | ||
| 035 | |a (NLM)28667437 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Fausther, Michel |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An Elf2-like transcription factor acts as repressor of the mouse ecto-5'-nucleotidase gene expression in hepatic myofibroblasts |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 27.07.2018 | ||
| 500 | |a Date Revised 30.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Hepatic fibrosis represents a pathological wound healing and tissue repair process triggered in response to chronic liver injury. A heterogeneous population of activated non-parenchymal liver cells, known as liver myofibroblasts, functions as the effector cells in hepatic fibrosis. Upon activation, liver myofibroblasts become fibrogenic, acquiring contractile properties and increasing collagen production capacity, while developing enhanced sensitivity to endogenous molecules and factors released in the local microenvironment. Hepatic extracellular adenosine is a bioactive small molecule, increasingly recognized as an important regulator of liver myofibroblast functions, and an important mediator in the pathogenesis of liver fibrosis overall. Remarkably, ecto-5'-nucleotidase/Nt5e/Cd73 enzyme, which accounts for the dominant adenosine-generating activity in the extracellular medium, is expressed by activated liver myofibroblasts. However, the molecular signals regulating Nt5e gene expression in liver myofibroblasts remain poorly understood. Here, we show that activated mouse liver myofibroblasts express Nt5e gene products and characterize the putative Nt5e minimal promoter in the mouse species. We describe the existence of an enhancer sequence upstream of the mouse Nt5e minimal promoter and establish that the mouse Nt5e minimal promoter transcriptional activity is negatively regulated by an Elf2-like Ets-related transcription factor in activated mouse liver myofibroblasts | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Adenosine | |
| 650 | 4 | |a Cloning | |
| 650 | 4 | |a Cluster of differentiation 73 (Cd73) | |
| 650 | 4 | |a E74-like factor 2/new ETS-related factor (Elf2/Nerf) | |
| 650 | 4 | |a ETS transcription factor family | |
| 650 | 4 | |a Ecto-5′-nucleotidase (Nt5e) | |
| 650 | 4 | |a Electric mobility shift assay | |
| 650 | 4 | |a Promoter | |
| 650 | 4 | |a Surface enzyme | |
| 650 | 4 | |a Transcriptional regulation | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a Elf2 protein, mouse |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a 5'-Nucleotidase |2 NLM | |
| 650 | 7 | |a EC 3.1.3.5 |2 NLM | |
| 700 | 1 | |a Lavoie, Elise G |e verfasserin |4 aut | |
| 700 | 1 | |a Goree, Jessica R |e verfasserin |4 aut | |
| 700 | 1 | |a Dranoff, Jonathan A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Purinergic signalling |d 2004 |g 13(2017), 4 vom: 01. Dez., Seite 417-428 |w (DE-627)NLM160620074 |x 1573-9546 |7 nnns |
| 773 | 1 | 8 | |g volume:13 |g year:2017 |g number:4 |g day:01 |g month:12 |g pages:417-428 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s11302-017-9570-7 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 13 |j 2017 |e 4 |b 01 |c 12 |h 417-428 | ||