Prediction and Analysis of Epitopes in clpP2 of Mycobacterium tuberculosis
OBJECTIVES: To predict and analyze the antigenic epitopes in Mycobacterium tuberculosis protein caseinolytic protease P2 (clpP2), and explore its possibility to be applied as a new tuberculosis (TB) vaccine and drug development target.
METHODS: Secondary structure of clpP2 based on nucleic sequence was predicted by DNA Star software. The homologous sequence conformation were analyzed by Swiss-Model online software. T cells antigenic epitopes were predicted through VaxiPred, and B cell epitopes were predicted by combining use of several different prediction programs, such as ABCpred, COBEPro and BepiPredPred. The immune characteristics of clpP2 were analyzed by DNA Star, SignalP, TMHMM online software and were searched through NCBI database.
RESULTS: clpP2protein was diverse in structure, composing with a great deal of CTL and Th cell epitopes. clpP2 was also predicted to comprise rich potential liner and discontinuous B-cell epitopes. These epitopes were accessible on the protein surface, located in flexible and hydrophilic regions.
CONCLUSION: clpP2 is prompted to induce immune responses and developes a novel target in surveillance, treatment and vaccine.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
|---|---|
| Enthalten in: |
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 48(2017), 2 vom: 21. März, Seite 244-247 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Liu, Si-Jing [VerfasserIn] |
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| Themen: |
Antigens, Bacterial |
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| Anmerkungen: |
Date Completed 28.05.2019 Date Revised 28.05.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM272916560 |
|---|
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|---|---|---|---|
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| 041 | |a chi | ||
| 100 | 1 | |a Liu, Si-Jing |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Prediction and Analysis of Epitopes in clpP2 of Mycobacterium tuberculosis |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 28.05.2019 | ||
| 500 | |a Date Revised 28.05.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: To predict and analyze the antigenic epitopes in Mycobacterium tuberculosis protein caseinolytic protease P2 (clpP2), and explore its possibility to be applied as a new tuberculosis (TB) vaccine and drug development target | ||
| 520 | |a METHODS: Secondary structure of clpP2 based on nucleic sequence was predicted by DNA Star software. The homologous sequence conformation were analyzed by Swiss-Model online software. T cells antigenic epitopes were predicted through VaxiPred, and B cell epitopes were predicted by combining use of several different prediction programs, such as ABCpred, COBEPro and BepiPredPred. The immune characteristics of clpP2 were analyzed by DNA Star, SignalP, TMHMM online software and were searched through NCBI database | ||
| 520 | |a RESULTS: clpP2protein was diverse in structure, composing with a great deal of CTL and Th cell epitopes. clpP2 was also predicted to comprise rich potential liner and discontinuous B-cell epitopes. These epitopes were accessible on the protein surface, located in flexible and hydrophilic regions | ||
| 520 | |a CONCLUSION: clpP2 is prompted to induce immune responses and developes a novel target in surveillance, treatment and vaccine | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Mycobacterium tuberculosis | |
| 650 | 4 | |a Epitope | |
| 650 | 4 | |a clpP2 | |
| 650 | 7 | |a Antigens, Bacterial |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Epitopes, T-Lymphocyte |2 NLM | |
| 650 | 7 | |a Tuberculosis Vaccines |2 NLM | |
| 650 | 7 | |a ClpP2 protein, bacteria |2 NLM | |
| 650 | 7 | |a EC 3.4.21.- |2 NLM | |
| 650 | 7 | |a Serine Endopeptidases |2 NLM | |
| 650 | 7 | |a EC 3.4.21.- |2 NLM | |
| 700 | 1 | |a Jiang, Ming-Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Pu, Qi-Kang |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Chen-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chuan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition |d 2003 |g 48(2017), 2 vom: 21. März, Seite 244-247 |w (DE-627)NLM126647062 |x 1672-173X |7 nnns |
| 773 | 1 | 8 | |g volume:48 |g year:2017 |g number:2 |g day:21 |g month:03 |g pages:244-247 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 48 |j 2017 |e 2 |b 21 |c 03 |h 244-247 | ||