Simultaneous Intranasal/Intravascular Antibody Labeling of CD4+ T Cells in Mouse Lungs
CD4+ T cell responses have been shown to be protective in many respiratory virus infections. In the respiratory tract, CD4+ T cells include cells in the airway and parenchyma and cells adhering to the pulmonary vasculature. Here we discuss in detail the methods that are useful for characterizing CD4+ T cells in different anatomic locations in mouse lungs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Bio-protocol - 7(2017), 1 vom: 05. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Yanqun [VerfasserIn] |
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| Links: |
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| Themen: |
Bronchoalveolar lavage fluid |
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| Anmerkungen: |
Date Revised 10.02.2022 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.21769/BioProtoc.2099 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM272005266 |
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| 520 | |a CD4+ T cell responses have been shown to be protective in many respiratory virus infections. In the respiratory tract, CD4+ T cells include cells in the airway and parenchyma and cells adhering to the pulmonary vasculature. Here we discuss in detail the methods that are useful for characterizing CD4+ T cells in different anatomic locations in mouse lungs | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Flow cytometry | |
| 650 | 4 | |a Intranasal/intravascular antibody labeling | |
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| 700 | 1 | |a Zhao, Jingxian |e verfasserin |4 aut | |
| 700 | 1 | |a Perlman, Stanley |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jincun |e verfasserin |4 aut | |
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