Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3'-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3'-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2017 |
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Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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Enthalten in: |
Frontiers of medicine - 11(2017), 2 vom: 01. Juni, Seite 214-222 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Zhou, Bo [VerfasserIn] |
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Links: |
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Themen: |
3' Untranslated Regions |
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Anmerkungen: |
Date Completed 20.04.2018 Date Revised 21.02.2019 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1007/s11684-017-0518-7 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM271560134 |
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245 | 1 | 0 | |a Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer |
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520 | |a MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3'-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3'-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer | ||
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700 | 1 | |a Xu, Hongbin |e verfasserin |4 aut | |
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700 | 1 | |a Guo, Ensong |e verfasserin |4 aut | |
700 | 1 | |a Guo, Lili |e verfasserin |4 aut | |
700 | 1 | |a Shan, Wanying |e verfasserin |4 aut | |
700 | 1 | |a Lu, Hao |e verfasserin |4 aut | |
700 | 1 | |a Wu, Yifan |e verfasserin |4 aut | |
700 | 1 | |a Li, Yuan |e verfasserin |4 aut | |
700 | 1 | |a Yang, Degui |e verfasserin |4 aut | |
700 | 1 | |a Weng, Danhui |e verfasserin |4 aut | |
700 | 1 | |a Meng, Li |e verfasserin |4 aut | |
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700 | 1 | |a Ma, Ding |e verfasserin |4 aut | |
700 | 1 | |a Chen, Gang |e verfasserin |4 aut | |
700 | 1 | |a Li, Kezhen |e verfasserin |4 aut | |
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