Anti-cancer Effect of Cyanidin-3-glucoside from Mulberry via Caspase-3 Cleavage and DNA Fragmentation in vitro and in vivo
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.org..
BACKGROUND: Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated.
METHODS: We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-methanol extraction methods. This molecule were compared mass spectroscopic properties by LC-MS/MS and analyzed by 1H and 13C NMR. We examined the anti-cancer effect with molecular mechanisms of the cyanidin-3-glucoside on MDA-MB-453 human breast cancer cells and xenograft animal model.
RESULTS: The treatment with the mulberry cyanidin-3-glucoside decreased cell viability in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, Treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibits tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was significantly reduced compared to the control group by the cyanidin-3-glucoside.
CONCLUSION: The data demonstrate that cyanidin-3-glucoside isolated from mulberry induced apoptosis in breast cancer (MDA-MB-453) cells, and therefore, has a potential as an anti-cancer agent. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in vitro and in vivo model and, indicating the inhibition of tumor progression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Anti-cancer agents in medicinal chemistry - 17(2017), 11 vom: 24. Nov., Seite 1519-1525 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cho, Eugene [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.12.2017 Date Revised 12.01.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.2174/1871520617666170327152026 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM270444629 |
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| 100 | 1 | |a Cho, Eugene |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Anti-cancer Effect of Cyanidin-3-glucoside from Mulberry via Caspase-3 Cleavage and DNA Fragmentation in vitro and in vivo |
| 264 | 1 | |c 2017 | |
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| 500 | |a Date Completed 25.12.2017 | ||
| 500 | |a Date Revised 12.01.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.org. | ||
| 520 | |a BACKGROUND: Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated | ||
| 520 | |a METHODS: We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-methanol extraction methods. This molecule were compared mass spectroscopic properties by LC-MS/MS and analyzed by 1H and 13C NMR. We examined the anti-cancer effect with molecular mechanisms of the cyanidin-3-glucoside on MDA-MB-453 human breast cancer cells and xenograft animal model | ||
| 520 | |a RESULTS: The treatment with the mulberry cyanidin-3-glucoside decreased cell viability in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, Treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibits tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was significantly reduced compared to the control group by the cyanidin-3-glucoside | ||
| 520 | |a CONCLUSION: The data demonstrate that cyanidin-3-glucoside isolated from mulberry induced apoptosis in breast cancer (MDA-MB-453) cells, and therefore, has a potential as an anti-cancer agent. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in vitro and in vivo model and, indicating the inhibition of tumor progression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cyanidin-3-glucoside | |
| 650 | 4 | |a DNA fragmentation | |
| 650 | 4 | |a Morus alba L. | |
| 650 | 4 | |a anti-cancer | |
| 650 | 4 | |a caspase-3 | |
| 650 | 4 | |a mulberry | |
| 650 | 4 | |a nude mice. | |
| 650 | 7 | |a Anthocyanins |2 NLM | |
| 650 | 7 | |a Antineoplastic Agents, Phytogenic |2 NLM | |
| 650 | 7 | |a Glucosides |2 NLM | |
| 650 | 7 | |a cyanidin-3-O-beta-glucopyranoside |2 NLM | |
| 650 | 7 | |a Caspase 3 |2 NLM | |
| 650 | 7 | |a EC 3.4.22.- |2 NLM | |
| 700 | 1 | |a Chung, Eun Y |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Hye-Yeon |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, On-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Chae, Hee S |e verfasserin |4 aut | |
| 700 | 1 | |a Jeong, Young-Ju |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Sun-Young |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Byeong-Soo |e verfasserin |4 aut | |
| 700 | 1 | |a Yoo, Dong J |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jong-Suk |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Kwang-Hyun |e verfasserin |4 aut | |
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