Protective ability against oxidative stress of brewers' spent grain protein hydrolysates
Copyright © 2017 Elsevier Ltd. All rights reserved..
The protein fraction of Brewers' spent grain (BSG) was used as substrate to obtain hydrolysates with antioxidant activity. Three enzymatic approaches were applied: brewer's spent yeast (BSY) proteases, Neutrase® and Alcalase®, at the same proteolytic activity (1U/mL), using an enzyme/substrate ratio of 10:100 (v/v), at 50°C, 4h. Total Phenolic Content (TPC) and Ferric Ion Reducing Antioxidant Power (FRAP) of hydrolysates and fractions <10kDa and <3kDa were assayed. Additionally, the protective ability of <10kDa fractions against oxidative stress on Caco-2 and HepG2 cells was investigated. Alcalase® hydrolysate presented significantly (p<0.05) higher TPC and FRAP (0.083mgGAE/mgdw; 0.101mgTE/mgdw, respectively) than Neutrase® and BSY hydrolysates. The three BSG protein hydrolysates (fraction <10kDa) exerted protective effect against free-radical induced cytotoxicity in Caco-2 and HepG2 cell lines, but the strongest effect was observed for BSY hydrolysates, therefore, it presents greater potential as functional ingredient.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:228 |
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| Enthalten in: |
Food chemistry - 228(2017) vom: 01. Aug., Seite 602-609 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vieira, Elsa F [VerfasserIn] |
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| Links: |
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| Themen: |
Alcalase® |
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| Anmerkungen: |
Date Completed 01.05.2017 Date Revised 10.04.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2017.02.050 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM270074767 |
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| 500 | |a Date Revised 10.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2017 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The protein fraction of Brewers' spent grain (BSG) was used as substrate to obtain hydrolysates with antioxidant activity. Three enzymatic approaches were applied: brewer's spent yeast (BSY) proteases, Neutrase® and Alcalase®, at the same proteolytic activity (1U/mL), using an enzyme/substrate ratio of 10:100 (v/v), at 50°C, 4h. Total Phenolic Content (TPC) and Ferric Ion Reducing Antioxidant Power (FRAP) of hydrolysates and fractions <10kDa and <3kDa were assayed. Additionally, the protective ability of <10kDa fractions against oxidative stress on Caco-2 and HepG2 cells was investigated. Alcalase® hydrolysate presented significantly (p<0.05) higher TPC and FRAP (0.083mgGAE/mgdw; 0.101mgTE/mgdw, respectively) than Neutrase® and BSY hydrolysates. The three BSG protein hydrolysates (fraction <10kDa) exerted protective effect against free-radical induced cytotoxicity in Caco-2 and HepG2 cell lines, but the strongest effect was observed for BSY hydrolysates, therefore, it presents greater potential as functional ingredient | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Alcalase® | |
| 650 | 4 | |a Antioxidant activity | |
| 650 | 4 | |a Brewer’s spent grain (BSG) | |
| 650 | 4 | |a Brewer’s spent yeast (BSY) | |
| 650 | 4 | |a Caco-2 cells | |
| 650 | 4 | |a HepG2 cells | |
| 650 | 4 | |a Neutrase® | |
| 650 | 4 | |a Protein hydrolysates | |
| 650 | 7 | |a Protein Hydrolysates |2 NLM | |
| 700 | 1 | |a da Silva, Diana Dias |e verfasserin |4 aut | |
| 700 | 1 | |a Carmo, Helena |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Isabel M P L V O |e verfasserin |4 aut | |
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