Prevalence and characteristics of pks genotoxin gene cluster-positive clinical Klebsiella pneumoniae isolates in Taiwan
The pks gene cluster encodes enzymes responsible for the synthesis of colibactin, a genotoxin that has been shown to induce DNA damage and contribute to increased virulence. The present study investigated the prevalence of pks in clinical K. pneumoniae isolates from a national surveillance program in Taiwan, and identified microbiological and molecular factors associated with pks-carriage. The pks gene cluster was detected in 67 (16.7%) of 400 isolates from various specimen types. Multivariate analysis revealed that isolates of K1, K2, K20, and K62 capsular types (p < 0.001), and those more susceptible to antimicrobial agents (p = 0.001) were independent factors strongly associated with pks-carriage. Phylogenetic studies on the sequence type (ST) and pulsed-field gel electrophoresis patterns indicated that the pks-positive isolates belong to a clonal group of ST23 in K1, a locally expanding ST65 clone in K2, a ST268-related K20 group, and a highly clonal ST36:K62 group. Carriage of rmpA, iutC, and ybtA, the genes associated with hypervirulence, was significantly higher in the pks-positive isolates than the pks-negative isolates (95.5% vs. 13.2%, p < 0.001). Further studies to determine the presence of hypervirulent pks-bearing bacterial populations in the flora of community residents and their association with different disease entities may be warranted.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Scientific reports - 7(2017) vom: 24. Feb., Seite 43120 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Ying-Tsong [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 18.12.2018 Date Revised 18.12.2018 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/srep43120 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM269259465 |
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| 245 | 1 | 0 | |a Prevalence and characteristics of pks genotoxin gene cluster-positive clinical Klebsiella pneumoniae isolates in Taiwan |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The pks gene cluster encodes enzymes responsible for the synthesis of colibactin, a genotoxin that has been shown to induce DNA damage and contribute to increased virulence. The present study investigated the prevalence of pks in clinical K. pneumoniae isolates from a national surveillance program in Taiwan, and identified microbiological and molecular factors associated with pks-carriage. The pks gene cluster was detected in 67 (16.7%) of 400 isolates from various specimen types. Multivariate analysis revealed that isolates of K1, K2, K20, and K62 capsular types (p < 0.001), and those more susceptible to antimicrobial agents (p = 0.001) were independent factors strongly associated with pks-carriage. Phylogenetic studies on the sequence type (ST) and pulsed-field gel electrophoresis patterns indicated that the pks-positive isolates belong to a clonal group of ST23 in K1, a locally expanding ST65 clone in K2, a ST268-related K20 group, and a highly clonal ST36:K62 group. Carriage of rmpA, iutC, and ybtA, the genes associated with hypervirulence, was significantly higher in the pks-positive isolates than the pks-negative isolates (95.5% vs. 13.2%, p < 0.001). Further studies to determine the presence of hypervirulent pks-bearing bacterial populations in the flora of community residents and their association with different disease entities may be warranted | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Polyketides |2 NLM | |
| 650 | 7 | |a Virulence Factors |2 NLM | |
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| 700 | 1 | |a Tan, Mei-Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Hsieh, Li-Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jann-Tay |e verfasserin |4 aut | |
| 700 | 1 | |a Shiau, Yih-Ru |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hui-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Ann-Chi |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Jui-Fen |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, I-Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Lauderdale, Tsai-Ling |e verfasserin |4 aut | |
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