A melamine based molecularly imprinted sensor for the determination of 8-hydroxydeoxyguanosine in human urine
Copyright © 2017 Elsevier B.V. All rights reserved..
A sensitive and facile molecular imprinted sensor has been fabricated using edge plane pyrolytic graphite (EPPG) for the determination of an important oxidative DNA damage product, 8-hydroxydeoxyguanosine (8-OHdG). The molecularly imprinted polymer film was fabricated by electropolymerization of melamine in the presence of 8-OHdG, on glutaraldehyde/poly 1,5-diaminonaphthalene modified EPPG. The imprinted sensor surface was characterized by using Field Emission Scanning Electron Microscopy, Electron Impedance Spectroscopy, Cyclic Voltammetry, Square Wave Voltammetry and UV-visible spectroscopy. The calibration response was linear over a concentration range of 20×10-9-3×10-6M of 8-OHdG with sensitivity and limit of detection (3σ/b) as 10.59µM/µA and 3×10-9M respectively. The common metabolites in urine, like uric acid, ascorbic acid, xanthine, hypoxanthine do not interfere up to 100-fold concentration. The imprinted sensor is also successfully employed for the determination of 8-OHdG in human urine sample of a renal failure patient.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:166 |
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| Enthalten in: |
Talanta - 166(2017) vom: 01. Mai, Seite 215-222 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kumar, Neeraj [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 05.09.2018 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2017.01.058 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Copyright © 2017 Elsevier B.V. All rights reserved. | ||
| 520 | |a A sensitive and facile molecular imprinted sensor has been fabricated using edge plane pyrolytic graphite (EPPG) for the determination of an important oxidative DNA damage product, 8-hydroxydeoxyguanosine (8-OHdG). The molecularly imprinted polymer film was fabricated by electropolymerization of melamine in the presence of 8-OHdG, on glutaraldehyde/poly 1,5-diaminonaphthalene modified EPPG. The imprinted sensor surface was characterized by using Field Emission Scanning Electron Microscopy, Electron Impedance Spectroscopy, Cyclic Voltammetry, Square Wave Voltammetry and UV-visible spectroscopy. The calibration response was linear over a concentration range of 20×10-9-3×10-6M of 8-OHdG with sensitivity and limit of detection (3σ/b) as 10.59µM/µA and 3×10-9M respectively. The common metabolites in urine, like uric acid, ascorbic acid, xanthine, hypoxanthine do not interfere up to 100-fold concentration. The imprinted sensor is also successfully employed for the determination of 8-OHdG in human urine sample of a renal failure patient | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 1,5-Diaminonaphthalene | |
| 650 | 4 | |a 8-Hydroxydeoxyguanosine | |
| 650 | 4 | |a Glutaraldehyde | |
| 650 | 4 | |a Human urine | |
| 650 | 4 | |a Melamine | |
| 650 | 4 | |a Molecular imprinted technique | |
| 650 | 7 | |a Polymers |2 NLM | |
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| 650 | 7 | |a Deoxyguanosine |2 NLM | |
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| 700 | 1 | |a Goyal, Rajendra N |e verfasserin |4 aut | |
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