Overexpression of SmeDEF Efflux Pump Decreases Aminoglycoside Resistance in Stenotrophomonas maltophilia
Copyright © 2017 American Society for Microbiology..
The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Antimicrobial agents and chemotherapy - 61(2017), 5 vom: 13. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Yi-Wei [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 02.01.2018 Date Revised 13.11.2018 published: Electronic-Print Citation Status MEDLINE |
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| doi: |
10.1128/AAC.02685-16 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM268921717 |
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| 245 | 1 | 0 | |a Overexpression of SmeDEF Efflux Pump Decreases Aminoglycoside Resistance in Stenotrophomonas maltophilia |
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| 520 | |a Copyright © 2017 American Society for Microbiology. | ||
| 520 | |a The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a antibiotic resistance | |
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| 650 | 4 | |a two-component regulatory system | |
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| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a SmeD protein, Stenotrophomonas maltophilia |2 NLM | |
| 650 | 7 | |a SmeE protein, Stenotrophomonas maltophilia |2 NLM | |
| 650 | 7 | |a SmeF protein, Stenotrophomonas maltophilia |2 NLM | |
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| 700 | 1 | |a Lin, Yi-Tsung |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Yi-Chih |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Tsuey-Ching |e verfasserin |4 aut | |
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