Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision
Copyright © 2017 Elsevier Inc. All rights reserved..
The life cycle of a primary cilium begins in quiescence and ends prior to mitosis. In quiescent cells, the primary cilium insulates itself from contiguous dynamic membrane processes on the cell surface to function as a stable signaling apparatus. Here, we demonstrate that basal restriction of ciliary structure dynamics is established by the cilia-enriched phosphoinositide 5-phosphatase, Inpp5e. Growth induction displaces ciliary Inpp5e and accumulates phosphatidylinositol 4,5-bisphosphate in distal cilia. This change triggers otherwise-forbidden actin polymerization in primary cilia, which excises cilia tips in a process we call cilia decapitation. While cilia disassembly is traditionally thought to occur solely through resorption, we show that an acute loss of IFT-B through cilia decapitation precedes resorption. Finally, we propose that cilia decapitation induces mitogenic signaling and constitutes a molecular link between the cilia life cycle and cell-division cycle. This newly defined ciliary mechanism may find significance in cell proliferation control during normal development and cancer.
| Errataetall: |
ErratumIn: Cell. 2019 Jun 27;178(1):261 Schurmans, Stéphane [added]. - PMID 31251914 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:168 |
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| Enthalten in: |
Cell - 168(2017), 1-2 vom: 12. Jan., Seite 264-279.e15 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Phua, Siew Cheng [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.05.2017 Date Revised 09.04.2022 published: Print-Electronic ErratumIn: Cell. 2019 Jun 27;178(1):261 Schurmans, Stéphane [added]. - PMID 31251914 Citation Status MEDLINE |
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| doi: |
10.1016/j.cell.2016.12.032 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM267985525 |
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| 500 | |a ErratumIn: Cell. 2019 Jun 27;178(1):261 Schurmans, Stéphane [added]. - PMID 31251914 | ||
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| 520 | |a The life cycle of a primary cilium begins in quiescence and ends prior to mitosis. In quiescent cells, the primary cilium insulates itself from contiguous dynamic membrane processes on the cell surface to function as a stable signaling apparatus. Here, we demonstrate that basal restriction of ciliary structure dynamics is established by the cilia-enriched phosphoinositide 5-phosphatase, Inpp5e. Growth induction displaces ciliary Inpp5e and accumulates phosphatidylinositol 4,5-bisphosphate in distal cilia. This change triggers otherwise-forbidden actin polymerization in primary cilia, which excises cilia tips in a process we call cilia decapitation. While cilia disassembly is traditionally thought to occur solely through resorption, we show that an acute loss of IFT-B through cilia decapitation precedes resorption. Finally, we propose that cilia decapitation induces mitogenic signaling and constitutes a molecular link between the cilia life cycle and cell-division cycle. This newly defined ciliary mechanism may find significance in cell proliferation control during normal development and cancer | ||
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| 700 | 1 | |a Su, Emily |e verfasserin |4 aut | |
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| 700 | 1 | |a Reiter, Jeremy F |e verfasserin |4 aut | |
| 700 | 1 | |a Ikegami, Koji |e verfasserin |4 aut | |
| 700 | 1 | |a Inoue, Takanari |e verfasserin |4 aut | |
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