Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats
BACKGROUND: Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2 (TLR2) is important for host recognition of invading Gram-positive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in vivo.
METHODS: We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria, triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1 (AP-1), phosphatidylinositol 3-kinase (PI3K) and transcription factor nuclear factor kappa B (NF-κB) and inflammation factors downstream of the signal pathway.
RESULTS: Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased mRNA levels of transcription factor NF-E2-related factor-2 (Nrf2) and the downstream gene, heme oxygenase-1 (HO-1). When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression level.
CONCLUSIONS: The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway.
| Errataetall: |
ErratumIn: J Anim Sci Biotechnol. 2017 Feb 15;8:19. - PMID 28239458 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Journal of animal science and biotechnology - 8(2017) vom: 15., Seite 3 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deng, Shoulong [VerfasserIn] |
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| Links: |
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| Themen: |
Haem oxygenase |
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| Anmerkungen: |
Date Revised 01.10.2020 published: Electronic-eCollection ErratumIn: J Anim Sci Biotechnol. 2017 Feb 15;8:19. - PMID 28239458 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1186/s40104-016-0136-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM267910614 |
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| 500 | |a ErratumIn: J Anim Sci Biotechnol. 2017 Feb 15;8:19. - PMID 28239458 | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2 (TLR2) is important for host recognition of invading Gram-positive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in vivo | ||
| 520 | |a METHODS: We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria, triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1 (AP-1), phosphatidylinositol 3-kinase (PI3K) and transcription factor nuclear factor kappa B (NF-κB) and inflammation factors downstream of the signal pathway | ||
| 520 | |a RESULTS: Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased mRNA levels of transcription factor NF-E2-related factor-2 (Nrf2) and the downstream gene, heme oxygenase-1 (HO-1). When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression level | ||
| 520 | |a CONCLUSIONS: The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Haem oxygenase | |
| 650 | 4 | |a Nrf2 signal pathway | |
| 650 | 4 | |a Toll-like receptor 2 | |
| 650 | 4 | |a Transgenic goats | |
| 700 | 1 | |a Yu, Kun |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Wuqi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shuotian |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Zhuo |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Yuchang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Baolu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Guoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yixun |e verfasserin |4 aut | |
| 700 | 1 | |a Lian, Zhengxing |e verfasserin |4 aut | |
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