Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State
Copyright © 2017 University of Oxford. Published by Elsevier Inc. All rights reserved..
One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Cell reports - 18(2017), 2 vom: 10. Jan., Seite 443-453 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jones, Daniel M [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.11.2017 Date Revised 10.01.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2016.12.032 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2017 University of Oxford. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion | ||
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| 700 | 1 | |a Alvarez, Luis A |e verfasserin |4 aut | |
| 700 | 1 | |a Nolan, Rory |e verfasserin |4 aut | |
| 700 | 1 | |a Ferriz, Margarita |e verfasserin |4 aut | |
| 700 | 1 | |a Sainz Urruela, Raquel |e verfasserin |4 aut | |
| 700 | 1 | |a Massana-Muñoz, Xènia |e verfasserin |4 aut | |
| 700 | 1 | |a Novak-Kotzer, Hila |e verfasserin |4 aut | |
| 700 | 1 | |a Dustin, Michael L |e verfasserin |4 aut | |
| 700 | 1 | |a Padilla-Parra, Sergi |e verfasserin |4 aut | |
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