Scout-MRM : Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection
Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:89 |
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| Enthalten in: |
Analytical chemistry - 89(2017), 3 vom: 07. Feb., Seite 1421-1426 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rougemont, Blandine [VerfasserIn] |
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| Anmerkungen: |
Date Completed 20.12.2018 Date Revised 09.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.analchem.6b03201 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM267520042 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Bontemps Gallo, Sébastien |e verfasserin |4 aut | |
| 700 | 1 | |a Ayciriex, Sophie |e verfasserin |4 aut | |
| 700 | 1 | |a Carrière, Romain |e verfasserin |4 aut | |
| 700 | 1 | |a Hondermarck, Hubert |e verfasserin |4 aut | |
| 700 | 1 | |a Lacroix, Jean Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Le Blanc, J C Yves |e verfasserin |4 aut | |
| 700 | 1 | |a Lemoine, Jérôme |e verfasserin |4 aut | |
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