The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells
Heat shock proteins Hsp) act as molecular chaperones, protecting enzymes and other proteins against reactive oxygen species. The objective of the study was to investigate the role of Hsp27 in maintaining the balance of the glutathione system and Hsp70 concentrations as well as in implementing Jurkat tumor cell apoptosis. Addition of the Hsp27 inhibitor KRIBB3 (5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazol) to Jurkat cells resulted in glutathione redox imbalance (increased GSSG and increased glutathione reductase activity), a decrease in Hsp70 concentrations, and also increased cell apoptosis as compared with to the intact cell culture. The proposed selective regulation of chaperone activity is a promising direction in regulating apoptosis at the cellular level.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:62 |
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| Enthalten in: |
Biomeditsinskaia khimiia - 62(2016), 6 vom: 08. Nov., Seite 670-673 |
| Sprache: |
Russisch |
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| Weiterer Titel: |
Uchastie belkov teplovogo shoka 27 i 70 v redoks-zavisimoĭ reguliatsii apoptoza opukholevykh kletok linii Jurkat |
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| Beteiligte Personen: |
Nosareva, O L [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.03.2017 Date Revised 09.12.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.18097/PBMC20166206670 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM26749842X |
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| 245 | 1 | 4 | |a The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells |
| 246 | 3 | 3 | |a Uchastie belkov teplovogo shoka 27 i 70 v redoks-zavisimoĭ reguliatsii apoptoza opukholevykh kletok linii Jurkat |
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| 520 | |a Heat shock proteins Hsp) act as molecular chaperones, protecting enzymes and other proteins against reactive oxygen species. The objective of the study was to investigate the role of Hsp27 in maintaining the balance of the glutathione system and Hsp70 concentrations as well as in implementing Jurkat tumor cell apoptosis. Addition of the Hsp27 inhibitor KRIBB3 (5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazol) to Jurkat cells resulted in glutathione redox imbalance (increased GSSG and increased glutathione reductase activity), a decrease in Hsp70 concentrations, and also increased cell apoptosis as compared with to the intact cell culture. The proposed selective regulation of chaperone activity is a promising direction in regulating apoptosis at the cellular level | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a cell redox status | |
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| 700 | 1 | |a Stepanova, E A |e verfasserin |4 aut | |
| 700 | 1 | |a Gulaya, V S |e verfasserin |4 aut | |
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