Crystal Structures of Bacterial (6-4) Photolyase Mutants with Impaired DNA Repair Activity
© 2016 The American Society of Photobiology..
PhrB from Agrobacterium fabrum is the first prokaryotic photolyase which repairs (6-4) UV DNA photoproducts. The protein harbors three cofactors: the enzymatically active FAD chromophore, a second chromophore, 6,7-dimethyl-8-ribityllumazine (DMRL) and a cubane-type Fe-S cluster. Tyr424 of PhrB is part of the DNA-binding site and could provide an electron link to the Fe-S cluster. The PhrBY424F mutant showed reduced binding of lesion DNA and loss of DNA repair. The mutant PhrBI51W is characterized by the loss of the DMRL chromophore, reduced photoreduction and reduced DNA repair capacity. We have determined the crystal structures of both mutants and found that both mutations only affect local protein environments, whereas the overall fold remained unchanged. The crystal structure of PhrBY424F revealed a water network extending to His366, which are part of the lesion-binding site. The crystal structure of PhrBI51W shows how the bulky Trp leads to structural rearrangements in the DMRL chromophore pocket. Spectral characterizations of PhrBI51W suggest that DMRL serves as an antenna chromophore for photoreduction and DNA repair in the wild type. The energy transfer from DMRL to FAD could represent a phylogenetically ancient process.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
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| Enthalten in: |
Photochemistry and photobiology - 93(2017), 1 vom: 01. Jan., Seite 304-314 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Fan [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 27.09.2018 Date Revised 27.09.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/php.12699 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM267271131 |
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| 520 | |a © 2016 The American Society of Photobiology. | ||
| 520 | |a PhrB from Agrobacterium fabrum is the first prokaryotic photolyase which repairs (6-4) UV DNA photoproducts. The protein harbors three cofactors: the enzymatically active FAD chromophore, a second chromophore, 6,7-dimethyl-8-ribityllumazine (DMRL) and a cubane-type Fe-S cluster. Tyr424 of PhrB is part of the DNA-binding site and could provide an electron link to the Fe-S cluster. The PhrBY424F mutant showed reduced binding of lesion DNA and loss of DNA repair. The mutant PhrBI51W is characterized by the loss of the DMRL chromophore, reduced photoreduction and reduced DNA repair capacity. We have determined the crystal structures of both mutants and found that both mutations only affect local protein environments, whereas the overall fold remained unchanged. The crystal structure of PhrBY424F revealed a water network extending to His366, which are part of the lesion-binding site. The crystal structure of PhrBI51W shows how the bulky Trp leads to structural rearrangements in the DMRL chromophore pocket. Spectral characterizations of PhrBI51W suggest that DMRL serves as an antenna chromophore for photoreduction and DNA repair in the wild type. The energy transfer from DMRL to FAD could represent a phylogenetically ancient process | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a pyrimidine(6-4)pyrimidone photolyase |2 NLM | |
| 650 | 7 | |a EC 4.1.99.- |2 NLM | |
| 650 | 7 | |a Deoxyribodipyrimidine Photo-Lyase |2 NLM | |
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| 700 | 1 | |a Ma, Hongju |e verfasserin |4 aut | |
| 700 | 1 | |a Bowatte, Kalinga |e verfasserin |4 aut | |
| 700 | 1 | |a Kwiatkowski, Dennis |e verfasserin |4 aut | |
| 700 | 1 | |a Mittmann, Esther |e verfasserin |4 aut | |
| 700 | 1 | |a Qasem, Heba |e verfasserin |4 aut | |
| 700 | 1 | |a Krauß, Norbert |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Xiaoli |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Scheerer, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiaojing |e verfasserin |4 aut | |
| 700 | 1 | |a Lamparter, Tilman |e verfasserin |4 aut | |
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