CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS
BACKGROUND: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers.
OBJECTIVE: This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae).
MATERIALS AND METHODS: The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes.
RESULTS: All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa.
CONCLUSION: Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Cryo letters - 37(2016), 4 vom: 20. Juli, Seite 264-271 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wayan Kurniani Karja, Ni [VerfasserIn] |
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| Themen: |
B8WCK70T7I |
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| Anmerkungen: |
Date Completed 16.06.2017 Date Revised 02.12.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM266885128 |
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| 041 | |a eng | ||
| 100 | 1 | |a Wayan Kurniani Karja, Ni |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 16.06.2017 | ||
| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers | ||
| 520 | |a OBJECTIVE: This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae) | ||
| 520 | |a MATERIALS AND METHODS: The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes | ||
| 520 | |a RESULTS: All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa | ||
| 520 | |a CONCLUSION: Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Fahrudin, Mokhamad |e verfasserin |4 aut | |
| 700 | 1 | |a Setiadi, Mohamad Agus |e verfasserin |4 aut | |
| 700 | 1 | |a Tumbelaka, Ligaya Ita |e verfasserin |4 aut | |
| 700 | 1 | |a Sudarwati, Retno |e verfasserin |4 aut | |
| 700 | 1 | |a Hastuti, Yohana Tri |e verfasserin |4 aut | |
| 700 | 1 | |a Mulia, Bongot Huas |e verfasserin |4 aut | |
| 700 | 1 | |a Widianti, Ardyta |e verfasserin |4 aut | |
| 700 | 1 | |a Sultan, Keni |e verfasserin |4 aut | |
| 700 | 1 | |a Terazono, Tsukasa |e verfasserin |4 aut | |
| 700 | 1 | |a Namula, Zhao |e verfasserin |4 aut | |
| 700 | 1 | |a Taniguchi, Masayasu |e verfasserin |4 aut | |
| 700 | 1 | |a Tanihara, Fuminori |e verfasserin |4 aut | |
| 700 | 1 | |a Takemoto, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Kikuchi, Kazuhiro |e verfasserin |4 aut | |
| 700 | 1 | |a Sato, Yoko |e verfasserin |4 aut | |
| 700 | 1 | |a Otoi, Takeshige |e verfasserin |4 aut | |
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