Effects of tripolyphosphate on cellular uptake and RNA interference efficiency of chitosan-based nanoparticles in Raw 264.7 macrophages
Copyright © 2016 Elsevier Inc. All rights reserved..
Tumor necrosis factor-α (TNF-α) is a major pro-inflammatory cytokine that is mainly secreted by macrophages during inflammation. Here, we synthesized a series of N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chlorides (HTCCs), and then used a complex coacervation technique or tripolyphosphate (TPP)-assisted ionotropic gelation strategy to complex the HTCCs with TNF-α siRNA (siTNF) to form nanoparticles (NPs). The resultant NPs had a desirable particle size (210-279nm), a slightly positive zeta potential (14-22mV), and negligible cytotoxicity against Raw 264.7 macrophages and colon-26 cells. Subsequent cellular uptake tests demonstrated that the introduction of TPP to the NPs markedly increased their cellular uptake efficiency (to nearly 100%) compared with TPP-free NPs, and yielded a correspondingly higher intracellular concentration of siRNA. Critically, in vitro gene silencing experiments revealed that all of the TPP-containing NPs showed excellent efficiency in inhibiting the mRNA expression level of TNF-α (by approximately 85-92%, which was much higher than that obtained using Oligofectamine/siTNF complexes). Collectively, these results obviously suggest that our non-toxic TPP-containing chitosan-based NPs can be exploited as efficient siTNF carriers for the treatment of inflammatory diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:490 |
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| Enthalten in: |
Journal of colloid and interface science - 490(2017) vom: 15. März, Seite 520-528 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Bo [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.04.2017 Date Revised 12.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jcis.2016.11.088 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Inc. All rights reserved. | ||
| 520 | |a Tumor necrosis factor-α (TNF-α) is a major pro-inflammatory cytokine that is mainly secreted by macrophages during inflammation. Here, we synthesized a series of N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chlorides (HTCCs), and then used a complex coacervation technique or tripolyphosphate (TPP)-assisted ionotropic gelation strategy to complex the HTCCs with TNF-α siRNA (siTNF) to form nanoparticles (NPs). The resultant NPs had a desirable particle size (210-279nm), a slightly positive zeta potential (14-22mV), and negligible cytotoxicity against Raw 264.7 macrophages and colon-26 cells. Subsequent cellular uptake tests demonstrated that the introduction of TPP to the NPs markedly increased their cellular uptake efficiency (to nearly 100%) compared with TPP-free NPs, and yielded a correspondingly higher intracellular concentration of siRNA. Critically, in vitro gene silencing experiments revealed that all of the TPP-containing NPs showed excellent efficiency in inhibiting the mRNA expression level of TNF-α (by approximately 85-92%, which was much higher than that obtained using Oligofectamine/siTNF complexes). Collectively, these results obviously suggest that our non-toxic TPP-containing chitosan-based NPs can be exploited as efficient siTNF carriers for the treatment of inflammatory diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chitosan | |
| 650 | 4 | |a Macrophage | |
| 650 | 4 | |a N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chloride | |
| 650 | 4 | |a Nanoparticle | |
| 650 | 4 | |a RNA interference | |
| 650 | 4 | |a Tripolyphosphate | |
| 650 | 7 | |a N-(2-hydroxypropyl)-3-trimethylammonium chitosan |2 NLM | |
| 650 | 7 | |a Polyphosphates |2 NLM | |
| 650 | 7 | |a Quaternary Ammonium Compounds |2 NLM | |
| 650 | 7 | |a RNA, Small Interfering |2 NLM | |
| 650 | 7 | |a Tumor Necrosis Factor-alpha |2 NLM | |
| 650 | 7 | |a Chitosan |2 NLM | |
| 650 | 7 | |a 9012-76-4 |2 NLM | |
| 650 | 7 | |a triphosphoric acid |2 NLM | |
| 650 | 7 | |a NU43IAG5BC |2 NLM | |
| 700 | 1 | |a Ma, Panpan |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Lijun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Qiubing |e verfasserin |4 aut | |
| 700 | 1 | |a Si, Xiaoying |e verfasserin |4 aut | |
| 700 | 1 | |a Walter, Lewins |e verfasserin |4 aut | |
| 700 | 1 | |a Merlin, Didier |e verfasserin |4 aut | |
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