Extraction of high-quality RNA from human articular cartilage
Copyright © 2016 Elsevier Inc. All rights reserved..
Extracting high-quality RNA from articular cartilage is challenging due to low cellularity and high proteoglycan content. This problem hinders efficient application of RNA sequencing (RNA-seq) analysis in studying cartilage homeostasis. Here we developed a method that purifies high-quality RNA directly from cartilage. Our method optimized the collection and homogenization steps so as to minimize RNA degradation, and modified the conventional TRIzol protocol to enhance RNA purity. Cartilage RNA purified using our method has appropriate quality for RNA-seq experiments including an RNA integrity number of ∼8. Our method also proved efficient in extracting high-quality RNA from subchondral bone.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:518 |
|---|---|
| Enthalten in: |
Analytical biochemistry - 518(2017) vom: 01. Feb., Seite 134-138 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Le Bleu, Heather K [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 08.05.2017 Date Revised 13.11.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ab.2016.11.018 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM266779247 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM266779247 | ||
| 003 | DE-627 | ||
| 005 | 20231224215252.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ab.2016.11.018 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0889.xml |
| 035 | |a (DE-627)NLM266779247 | ||
| 035 | |a (NLM)27913164 | ||
| 035 | |a (PII)S0003-2697(16)30401-8 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Le Bleu, Heather K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Extraction of high-quality RNA from human articular cartilage |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.05.2017 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Inc. All rights reserved. | ||
| 520 | |a Extracting high-quality RNA from articular cartilage is challenging due to low cellularity and high proteoglycan content. This problem hinders efficient application of RNA sequencing (RNA-seq) analysis in studying cartilage homeostasis. Here we developed a method that purifies high-quality RNA directly from cartilage. Our method optimized the collection and homogenization steps so as to minimize RNA degradation, and modified the conventional TRIzol protocol to enhance RNA purity. Cartilage RNA purified using our method has appropriate quality for RNA-seq experiments including an RNA integrity number of ∼8. Our method also proved efficient in extracting high-quality RNA from subchondral bone | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a RNA |2 NLM | |
| 650 | 7 | |a 63231-63-0 |2 NLM | |
| 700 | 1 | |a Kamal, Fadia A |e verfasserin |4 aut | |
| 700 | 1 | |a Kelly, Meghan |e verfasserin |4 aut | |
| 700 | 1 | |a Ketz, John P |e verfasserin |4 aut | |
| 700 | 1 | |a Zuscik, Michael J |e verfasserin |4 aut | |
| 700 | 1 | |a Elbarbary, Reyad A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical biochemistry |d 1960 |g 518(2017) vom: 01. Feb., Seite 134-138 |w (DE-627)NLM000012556 |x 1096-0309 |7 nnns |
| 773 | 1 | 8 | |g volume:518 |g year:2017 |g day:01 |g month:02 |g pages:134-138 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ab.2016.11.018 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 518 |j 2017 |b 01 |c 02 |h 134-138 | ||