MicroRNA-100 inhibits bone morphogenetic protein-induced osteoblast differentiation by targeting Smad1
OBJECTIVE: MicroRNAs (miRNAs) act as key regulators of diverse cellular activities by regulating the expression of protein-coding genes. Osteoblast differentiation, a fundamental step in skeletal development, involves the activation of several signaling pathways, including transforming growth factor β (TGF-β), bone morphogenetic protein (BMP), and Wnt signaling pathways.
MATERIALS AND METHODS: miRNA expression was measured using TaqManRT-PCR. Western blot was used to detect the protein expression of Smad1. Luciferase reporter assay was used to measure the luciferase activity.
RESULTS: In this study, we found that miR-100 was expressed in mesenchymal progenitor cell lines; furthermore, its expression was reduced during osteoblast differentiation. Retroviral overexpression of miR-100 decreased Smad1 protein levels, whereas miR-100 inhibition had the opposite effect, suggesting that miR-100 acts as an endogenous attenuator of Smad1 in osteoblast differentiation.
CONCLUSIONS: Together, our data demonstrate that miR-100 acts as an important endogenous negative regulator of BMP-induced osteoblast differentiation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
|---|---|
| Enthalten in: |
European review for medical and pharmacological sciences - 20(2016), 18 vom: 01. Sept., Seite 3911-3919 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fu, H-L [VerfasserIn] |
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| Themen: |
Bone Morphogenetic Proteins |
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| Anmerkungen: |
Date Completed 07.08.2017 Date Revised 18.03.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM265259061 |
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| 100 | 1 | |a Fu, H-L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a MicroRNA-100 inhibits bone morphogenetic protein-induced osteoblast differentiation by targeting Smad1 |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 07.08.2017 | ||
| 500 | |a Date Revised 18.03.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: MicroRNAs (miRNAs) act as key regulators of diverse cellular activities by regulating the expression of protein-coding genes. Osteoblast differentiation, a fundamental step in skeletal development, involves the activation of several signaling pathways, including transforming growth factor β (TGF-β), bone morphogenetic protein (BMP), and Wnt signaling pathways | ||
| 520 | |a MATERIALS AND METHODS: miRNA expression was measured using TaqManRT-PCR. Western blot was used to detect the protein expression of Smad1. Luciferase reporter assay was used to measure the luciferase activity | ||
| 520 | |a RESULTS: In this study, we found that miR-100 was expressed in mesenchymal progenitor cell lines; furthermore, its expression was reduced during osteoblast differentiation. Retroviral overexpression of miR-100 decreased Smad1 protein levels, whereas miR-100 inhibition had the opposite effect, suggesting that miR-100 acts as an endogenous attenuator of Smad1 in osteoblast differentiation | ||
| 520 | |a CONCLUSIONS: Together, our data demonstrate that miR-100 acts as an important endogenous negative regulator of BMP-induced osteoblast differentiation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Bone Morphogenetic Proteins |2 NLM | |
| 650 | 7 | |a MIRN100 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a Pan, H-X |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, B |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, B-C |e verfasserin |4 aut | |
| 700 | 1 | |a Shao, L |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, G-S |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Q |e verfasserin |4 aut | |
| 700 | 1 | |a Li, M |e verfasserin |4 aut | |
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