Coarse-grained simulations of hemolytic peptide δ-lysin interacting with a POPC bilayer
Copyright © 2016 Elsevier B.V. All rights reserved..
δ-lysin, secreted by a Gram-positive bacterium Staphylococcus aureus, is a 26-residue membrane active peptide that shares many common features with antimicrobial peptides (AMPs). However, it possesses a few unique features that differentiate itself from typical AMPs. In particular, δ-lysin has zero net charge, even though it has many charged residues, and it preferentially lyses eukaryotic cells over bacterial cells. Here, we present the results of coarse-grained molecular dynamics simulations of δ-lysin interacting with a zwitterionic membrane over a wide range of peptide concentrations. When the peptides concentration is low, spontaneous dimerization of peptides is observed on the membrane surface, but deep insertion of peptides or pore formation was not observed. However, the calculated free energy of peptide insertion suggests that a small fraction of peptides is likely to be present inside the membrane at the peptide concentrations typically seen in dye efflux experiments. When the simulations with multiple peptides are carried out with a single pre-inserted transmembrane peptide, spontaneous pore formation occurs with a peptide-to-lipid ratio (P/L) as low as P/L=1:42. Inter-peptide salt bridges among the transmembrane peptides seem to play a role in creating compact pores with very low level of hydration. More importantly, the transmembrane peptides making up the pore are constantly pushed to the opposite side of the membrane when the mass imbalance between the two sides of membrane is significant. Thus, the pore is very dynamic, allowing multiple peptides to translocate across the membrane simultaneously.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1858 |
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| Enthalten in: |
Biochimica et biophysica acta - 1858(2016), 12 vom: 26. Dez., Seite 3182-3194 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
King, Mariah J [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.10.2017 Date Revised 13.11.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbamem.2016.10.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM265140218 |
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| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a δ-lysin, secreted by a Gram-positive bacterium Staphylococcus aureus, is a 26-residue membrane active peptide that shares many common features with antimicrobial peptides (AMPs). However, it possesses a few unique features that differentiate itself from typical AMPs. In particular, δ-lysin has zero net charge, even though it has many charged residues, and it preferentially lyses eukaryotic cells over bacterial cells. Here, we present the results of coarse-grained molecular dynamics simulations of δ-lysin interacting with a zwitterionic membrane over a wide range of peptide concentrations. When the peptides concentration is low, spontaneous dimerization of peptides is observed on the membrane surface, but deep insertion of peptides or pore formation was not observed. However, the calculated free energy of peptide insertion suggests that a small fraction of peptides is likely to be present inside the membrane at the peptide concentrations typically seen in dye efflux experiments. When the simulations with multiple peptides are carried out with a single pre-inserted transmembrane peptide, spontaneous pore formation occurs with a peptide-to-lipid ratio (P/L) as low as P/L=1:42. Inter-peptide salt bridges among the transmembrane peptides seem to play a role in creating compact pores with very low level of hydration. More importantly, the transmembrane peptides making up the pore are constantly pushed to the opposite side of the membrane when the mass imbalance between the two sides of membrane is significant. Thus, the pore is very dynamic, allowing multiple peptides to translocate across the membrane simultaneously | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Coarse-grained simulation | |
| 650 | 4 | |a POPC bilayer | |
| 650 | 4 | |a Peptide dimerization | |
| 650 | 4 | |a Pore formation | |
| 650 | 4 | |a δ-Lysin | |
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| 650 | 7 | |a Hemolysin Proteins |2 NLM | |
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| 650 | 7 | |a Phosphatidylcholines |2 NLM | |
| 650 | 7 | |a delta hemolysin protein, Staphylococcus aureus |2 NLM | |
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| 650 | 7 | |a 1-palmitoyl-2-oleoylphosphatidylcholine |2 NLM | |
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| 700 | 1 | |a Bennett, Ashley L |e verfasserin |4 aut | |
| 700 | 1 | |a Almeida, Paulo F |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Hee-Seung |e verfasserin |4 aut | |
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