Development of shell cross-linked nanoparticles based on boronic acid-related reactions for self-regulated insulin delivery
Shell cross-linked nanoparticles were fabricated by the complexation of poly(3-methacrylamido phenylboronic acid) (PMAPBA) and thiolated chitosan (chitosan-SH) via boronic acid-related reactions. The formation of PMAPBA/chitosan-SH nanoparticles was confirmed by transmission electron microscopy, dynamic light scattering, and UV spectroscopy. The nanoparticles had a narrow size distribution with a relatively high positive charge density, and the size and zeta potential of the nanoparticles correlated with the chitosan-SH concentration. Furthermore, owing to the cross-linking of the nanoparticle shell, insulin was encapsulated in the nanoparticles with a loading capacity of up to 18%. The release of insulin from the nanoparticles slowed down because of the presence of disulfide bonds and increased with increasing glucose level in the medium. The structure of the released insulin was not distorted. More importantly, the nanoparticles had good cytocompatibility, as demonstrated by in vitro experiments. The simplicity of this strategy along with a high loading capacity, glucose sensitivity, and cytocompatibility of the produced nanoparticles should significantly boost their application in self-regulated insulin delivery.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
|---|---|
| Enthalten in: |
Journal of biomaterials science. Polymer edition - 28(2017), 1 vom: 01. Jan., Seite 93-106 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Yanxia [VerfasserIn] |
|---|
| Themen: |
9012-76-4 |
|---|
| Anmerkungen: |
Date Completed 12.12.2017 Date Revised 08.03.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM265130042 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM265130042 | ||
| 003 | DE-627 | ||
| 005 | 20231224211616.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2017 xx |||||o 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0883.xml |
| 035 | |a (DE-627)NLM265130042 | ||
| 035 | |a (NLM)27719550 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Yanxia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of shell cross-linked nanoparticles based on boronic acid-related reactions for self-regulated insulin delivery |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 12.12.2017 | ||
| 500 | |a Date Revised 08.03.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Shell cross-linked nanoparticles were fabricated by the complexation of poly(3-methacrylamido phenylboronic acid) (PMAPBA) and thiolated chitosan (chitosan-SH) via boronic acid-related reactions. The formation of PMAPBA/chitosan-SH nanoparticles was confirmed by transmission electron microscopy, dynamic light scattering, and UV spectroscopy. The nanoparticles had a narrow size distribution with a relatively high positive charge density, and the size and zeta potential of the nanoparticles correlated with the chitosan-SH concentration. Furthermore, owing to the cross-linking of the nanoparticle shell, insulin was encapsulated in the nanoparticles with a loading capacity of up to 18%. The release of insulin from the nanoparticles slowed down because of the presence of disulfide bonds and increased with increasing glucose level in the medium. The structure of the released insulin was not distorted. More importantly, the nanoparticles had good cytocompatibility, as demonstrated by in vitro experiments. The simplicity of this strategy along with a high loading capacity, glucose sensitivity, and cytocompatibility of the produced nanoparticles should significantly boost their application in self-regulated insulin delivery | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Thiolated chitosan | |
| 650 | 4 | |a glucose-sensitivity | |
| 650 | 4 | |a insulin delivery | |
| 650 | 4 | |a phenylboronic acids | |
| 650 | 4 | |a smart nanoparticles | |
| 650 | 7 | |a Boronic Acids |2 NLM | |
| 650 | 7 | |a Drug Carriers |2 NLM | |
| 650 | 7 | |a Insulin |2 NLM | |
| 650 | 7 | |a Chitosan |2 NLM | |
| 650 | 7 | |a 9012-76-4 |2 NLM | |
| 700 | 1 | |a Huang, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yingjuan |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Chai, Zhihua |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of biomaterials science. Polymer edition |d 1996 |g 28(2017), 1 vom: 01. Jan., Seite 93-106 |w (DE-627)NLM012635170 |x 1568-5624 |7 nnns |
| 773 | 1 | 8 | |g volume:28 |g year:2017 |g number:1 |g day:01 |g month:01 |g pages:93-106 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 28 |j 2017 |e 1 |b 01 |c 01 |h 93-106 | ||