Enhancement of liposome mediated gene transfer by adding cholesterol and cholesterol modulating drugs
Copyright © 2016 Elsevier B.V. All rights reserved..
Cholesterol is an important cell membrane component and has been used as co-lipid for cationic liposome to enhance gene delivery. However, the role of cholesterol in transfection efficiency has not been fully understood. In this study, transfection efficiency of liposome was measured after cholesterol was added to the cell culture medium. As a result, addition of cholesterol increased transfection efficiency of several liposomes consisting of different lipid components in various cells (AGS, CHO, COS7 and, MCF7). Furthermore, treatment of cells with cholesterol modulating drugs, imipramine and U18666A, also increased transfection efficiency of liposomes. To elucidate the role of added cholesterol in gene transfer, endocytotic mechanism was studied and also revealed that adding cholesterol in culture media induced participation of caveolae-mediated endocytosis and micropinocytosis in CHO cell. Therefore, the results of this work suggest that modulation of intracellular cholesterol can be an important method to enhance gene delivery.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1858 |
|---|---|
| Enthalten in: |
Biochimica et biophysica acta - 1858(2016), 12 vom: 19. Dez., Seite 3017-3023 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Bae, Yun-Ui [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one |
|---|
| Anmerkungen: |
Date Completed 26.10.2017 Date Revised 29.04.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.bbamem.2016.09.013 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM264658817 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM264658817 | ||
| 003 | DE-627 | ||
| 005 | 20231224210600.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bbamem.2016.09.013 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0882.xml |
| 035 | |a (DE-627)NLM264658817 | ||
| 035 | |a (NLM)27664498 | ||
| 035 | |a (PII)S0005-2736(16)30311-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bae, Yun-Ui |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Enhancement of liposome mediated gene transfer by adding cholesterol and cholesterol modulating drugs |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.10.2017 | ||
| 500 | |a Date Revised 29.04.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a Cholesterol is an important cell membrane component and has been used as co-lipid for cationic liposome to enhance gene delivery. However, the role of cholesterol in transfection efficiency has not been fully understood. In this study, transfection efficiency of liposome was measured after cholesterol was added to the cell culture medium. As a result, addition of cholesterol increased transfection efficiency of several liposomes consisting of different lipid components in various cells (AGS, CHO, COS7 and, MCF7). Furthermore, treatment of cells with cholesterol modulating drugs, imipramine and U18666A, also increased transfection efficiency of liposomes. To elucidate the role of added cholesterol in gene transfer, endocytotic mechanism was studied and also revealed that adding cholesterol in culture media induced participation of caveolae-mediated endocytosis and micropinocytosis in CHO cell. Therefore, the results of this work suggest that modulation of intracellular cholesterol can be an important method to enhance gene delivery | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cholesterol | |
| 650 | 4 | |a Gene therapy | |
| 650 | 4 | |a Liposomes | |
| 650 | 4 | |a Transfection | |
| 650 | 7 | |a Androstenes |2 NLM | |
| 650 | 7 | |a Liposomes |2 NLM | |
| 650 | 7 | |a 3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one |2 NLM | |
| 650 | 7 | |a 3039-71-2 |2 NLM | |
| 650 | 7 | |a Cholesterol |2 NLM | |
| 650 | 7 | |a 97C5T2UQ7J |2 NLM | |
| 650 | 7 | |a Imipramine |2 NLM | |
| 650 | 7 | |a OGG85SX4E4 |2 NLM | |
| 700 | 1 | |a Huh, Jae-Wan |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Bieong-Kil |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Hyeon Young |e verfasserin |4 aut | |
| 700 | 1 | |a Seu, Young-Bae |e verfasserin |4 aut | |
| 700 | 1 | |a Doh, Kyung-Oh |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochimica et biophysica acta |d 1948 |g 1858(2016), 12 vom: 19. Dez., Seite 3017-3023 |w (DE-627)NLM000000698 |x 0006-3002 |7 nnns |
| 773 | 1 | 8 | |g volume:1858 |g year:2016 |g number:12 |g day:19 |g month:12 |g pages:3017-3023 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bbamem.2016.09.013 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 1858 |j 2016 |e 12 |b 19 |c 12 |h 3017-3023 | ||