An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04
Copyright © 2016. Published by Elsevier B.V..
A novel laccase was isolated from fermentation broth of the mycorrhizal fungus Leucoagaricus naucinus LAC-04 by using a protocol that comprising ion-exchange chromatography steps on DEAE-cellulose, SP-Sepharose, and Q-Sepharose, and finally gel filtration by fast protein liquid chromatography on Superdex 75. The laccase (LNL) was purified with a purification fold of 21.19 and a recovery rate of 19.8%. It is a monomeric protein with a molecular mass of 56kDa. LNL lacks absorption around 600nm, which indicates that the purified laccase is a yellow laccases. LNL demonstrates an optimal pH of 2.2 and an optimal temperature range of 30-60°C using ABTS as the substrate. It is inhibited in the presence of EDTA and metal ions including Cd2+, Co2+, Cu2+. The Km of the laccase towards ABTS is estimated to 50.12μM at pH 2.2 and 30°C. Moreover, the purified laccase manifests effective decolorizing activity towards azo, heterocyclic, and aromatic dyes including Bromothymol Blue, Eriochrome Black T, Evans Bue, Fuchsin Basic, and Remazol Brilliant Blue R.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
|---|---|
| Enthalten in: |
International journal of biological macromolecules - 93(2016), Pt A vom: 01. Dez., Seite 837-842 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ning, Ying-Jie [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Coloring Agents |
|---|
| Anmerkungen: |
Date Completed 17.03.2017 Date Revised 17.03.2017 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ijbiomac.2016.09.046 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM264456955 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM264456955 | ||
| 003 | DE-627 | ||
| 005 | 20231224210130.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ijbiomac.2016.09.046 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0881.xml |
| 035 | |a (DE-627)NLM264456955 | ||
| 035 | |a (NLM)27642125 | ||
| 035 | |a (PII)S0141-8130(16)31636-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ning, Ying-Jie |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04 |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 17.03.2017 | ||
| 500 | |a Date Revised 17.03.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016. Published by Elsevier B.V. | ||
| 520 | |a A novel laccase was isolated from fermentation broth of the mycorrhizal fungus Leucoagaricus naucinus LAC-04 by using a protocol that comprising ion-exchange chromatography steps on DEAE-cellulose, SP-Sepharose, and Q-Sepharose, and finally gel filtration by fast protein liquid chromatography on Superdex 75. The laccase (LNL) was purified with a purification fold of 21.19 and a recovery rate of 19.8%. It is a monomeric protein with a molecular mass of 56kDa. LNL lacks absorption around 600nm, which indicates that the purified laccase is a yellow laccases. LNL demonstrates an optimal pH of 2.2 and an optimal temperature range of 30-60°C using ABTS as the substrate. It is inhibited in the presence of EDTA and metal ions including Cd2+, Co2+, Cu2+. The Km of the laccase towards ABTS is estimated to 50.12μM at pH 2.2 and 30°C. Moreover, the purified laccase manifests effective decolorizing activity towards azo, heterocyclic, and aromatic dyes including Bromothymol Blue, Eriochrome Black T, Evans Bue, Fuchsin Basic, and Remazol Brilliant Blue R | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dye decolorizing activity | |
| 650 | 4 | |a Laccase | |
| 650 | 4 | |a Leucoagaricus naucinus | |
| 650 | 7 | |a Coloring Agents |2 NLM | |
| 650 | 7 | |a Fungal Proteins |2 NLM | |
| 650 | 7 | |a Laccase |2 NLM | |
| 650 | 7 | |a EC 1.10.3.2 |2 NLM | |
| 700 | 1 | |a Wang, Shan-Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Qing-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Ling, Zhuo-Ren |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shou-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wen-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guo-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Meng-Juan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of biological macromolecules |d 1992 |g 93(2016), Pt A vom: 01. Dez., Seite 837-842 |w (DE-627)NLM012627356 |x 1879-0003 |7 nnns |
| 773 | 1 | 8 | |g volume:93 |g year:2016 |g number:Pt A |g day:01 |g month:12 |g pages:837-842 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ijbiomac.2016.09.046 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 93 |j 2016 |e Pt A |b 01 |c 12 |h 837-842 | ||