Levels of DNA damage in peripheral blood lymphocytes of patients undergoing standard hemodialysis vs on-line hemodiafiltration : A comet assay investigation
Copyright © 2016 Elsevier B.V. All rights reserved..
Chronic kidney disease (CKD) patients exhibit high levels of genetic damage. Part of this genetic damage is supposed to be caused by the hemodialysis (HD) therapy. Different and more efficient HD procedures could reduce the genetic damage and improve health status of CKD patients. In the present study, we analyzed if changing to online hemodiafiltration (OL-HDF) has a beneficial effect on the levels of genetic damage. The levels of genetic damage (DNA breaks and oxidatively damaged DNA) were analyzed in peripheral blood lymphocytes by using the comet assay. Forty-nine patients submitted to HD, 34 of them changing to OL-HDF and 15 patients continuing in low-flux HD, were included in the study. Plasma antioxidant capacity was also determined. Second sampling period was established after 6 months on the new or traditional HD protocol. A slight decrease in the levels of DNA damage was observed in patients who switched to OL-HDF (P=0.048) in relation to the reference group. This reduction is indicative that OL-HDF shows greater efficiency than low-flux HD in the reduction of basal levels of genetic damage.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:808 |
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| Enthalten in: |
Mutation research. Genetic toxicology and environmental mutagenesis - 808(2016) vom: 15. Sept., Seite 1-7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Corredor, Zuray [VerfasserIn] |
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| Links: |
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| Themen: |
Chronic kidney disease |
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| Anmerkungen: |
Date Completed 27.01.2017 Date Revised 27.01.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.mrgentox.2016.07.008 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM264417488 |
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| 245 | 1 | 0 | |a Levels of DNA damage in peripheral blood lymphocytes of patients undergoing standard hemodialysis vs on-line hemodiafiltration |b A comet assay investigation |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a Chronic kidney disease (CKD) patients exhibit high levels of genetic damage. Part of this genetic damage is supposed to be caused by the hemodialysis (HD) therapy. Different and more efficient HD procedures could reduce the genetic damage and improve health status of CKD patients. In the present study, we analyzed if changing to online hemodiafiltration (OL-HDF) has a beneficial effect on the levels of genetic damage. The levels of genetic damage (DNA breaks and oxidatively damaged DNA) were analyzed in peripheral blood lymphocytes by using the comet assay. Forty-nine patients submitted to HD, 34 of them changing to OL-HDF and 15 patients continuing in low-flux HD, were included in the study. Plasma antioxidant capacity was also determined. Second sampling period was established after 6 months on the new or traditional HD protocol. A slight decrease in the levels of DNA damage was observed in patients who switched to OL-HDF (P=0.048) in relation to the reference group. This reduction is indicative that OL-HDF shows greater efficiency than low-flux HD in the reduction of basal levels of genetic damage | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chronic kidney disease | |
| 650 | 4 | |a Comet assay | |
| 650 | 4 | |a On-line hemodiafiltration | |
| 650 | 4 | |a Peripheral blood lymphocytes | |
| 700 | 1 | |a Rodríguez-Ribera, Lara |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Irene |e verfasserin |4 aut | |
| 700 | 1 | |a Díaz, Juan Manuel |e verfasserin |4 aut | |
| 700 | 1 | |a Ballarín, José |e verfasserin |4 aut | |
| 700 | 1 | |a Marcos, Ricard |e verfasserin |4 aut | |
| 700 | 1 | |a Coll, Elisabet |e verfasserin |4 aut | |
| 700 | 1 | |a Pastor, Susana |e verfasserin |4 aut | |
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